LOCUS       sequence-494452-       10404 bp    DNA     circular SYN 02-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of human RECK (all-in-one
            Tet-On system), N-terminal BAP tag .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Heiden R, Hannig L, Kuo CJ, Ergun S, Braunger BM, Vallon M
  TITLE     WNT7A/B assemble a GPR124-RECK-LRP5/6 co-receptor complex to 
            activate beta-catenin signaling in brain endothelial cells.
  JOURNAL   J Biol Chem. 2025 Sep 4:110682. doi: 10.1016/j.jbc.2025.110682.
  PUBMED    40914247
REFERENCE   2  (bases 1 to 10404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 2, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2025 Sep 4:110682. doi: 10.1016/j.jbc.2025.110682."
COMMENT     Sequence Label: pCW-BAP-RECK-Blast
FEATURES             Location/Qualifiers
     source          1..10404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             624..668
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     promoter        3547..4057
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3976..3994
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4067..4462
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4463..4516
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4517..5134,5135..5140,5141..5176,5177..5179,5180..5215,
                     5216..5218,5219..5254,5255..5263)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5303..5323)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5721..5732)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5937..6170
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6242..6363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6279..6298)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6333..6352
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6403..6538
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6538..6609
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6610..6678,6679..7470)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6828..6847)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7641..8229
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8130..8149
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8383..8400
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8517..8538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8553..8558,8559..8576,8577..8583)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8591..8607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8596..8618
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8615..8631
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8615..8631
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8650..8670
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8652..8670
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8698..8924
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8925..9105
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9152..9277
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9770..10003
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10188..10232
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcaccatggc gaccgtccgg gcctctctgc gaggtgcgct gctccttctg ctggccgtgg
      601 cgggggtcgc ggaggtggca gggggtctga acgacatctt cgaggctcag aaaatcgaat
      661 ggcacgaggg cctggctccg ggcagtgcgg gtgcattgtg ttgtaatcat tcaaaggata
      721 accaaatgtg ccgtgatgta tgtgaacaga ttttctcctc aaaaagtgaa tcccgactaa
      781 aacatctgtt gcagcgagcc ccagattatt gcccagagac aatggttgaa atttggaatt
      841 gtatgaattc atctttgcca ggtgtgttta agaagtctga tggctgggtt ggcttaggct
      901 gctgtgaact ggctattgcc ttggagtgtc gacaggcatg caagcaggca tcttcaaaga
      961 atgatatttc caaagtttgc agaaaagaat atgagaatgc tcttttcagt tgcattagca
     1021 gaaatgaaat gggctcggtt tgttgcagtt atgcaggtca tcacacaaac tgccgagaat
     1081 actgtcaagc catttttcga acagactctt ctcctggtcc atctcagata aaagcagtgg
     1141 aaaattattg cgcctctatt agtccacaat taatacattg tgtgaacaat tatactcaat
     1201 cttatccaat gaggaaccca acggatagtt tatattgctg tgacagagct gaagaccatg
     1261 cttgccaaaa tgcctgcaag agaatcctga tgtccaagaa aacggaaatg gagattgttg
     1321 atggtctcat cgagggttgt aagacccagc ccttgcctca agatcctctt tggcaatgtt
     1381 ttcttgaaag ctcacaatct gttcaccctg gagtcactgt acaccctcct ccctctacag
     1441 gcctcgatgg ggctaaattg cattgttgtt ctaaagcaaa cacttcaaca tgtagggaac
     1501 tctgcactaa actttacagc atgagctggg gcaatacaca gagttggcaa gagtttgatc
     1561 gcttttgtga atataatcca gtggaagtgt ccatgttgac ctgtttagcg gatgtccggg
     1621 aaccttgcca gttgggctgt agaaacctta cttactgtac taattttaac aacaggccaa
     1681 cagaactttt caggagttgt aatgcacagt cagatcaagg agccatgaat gacatgaagt
     1741 tgtgggagaa aggaagcata aagatgccat ttatcaatat acctgttctt gatattaaaa
     1801 agtgccagcc agagatgtgg aaagcaatag cttgttcact gcagattaaa ccttgtcata
     1861 gtaaatctcg gggaagtatt atttgcaaat cagattgtgt ggagattctt aaaaaatgtg
     1921 gagaccagaa caaattccct gaagaccaca cagctgaaag tatttgtgag cttctgtcac
     1981 ctacagatga tctgaagaat tgtatacctt tggatacata cctcaggcca agtactttag
     2041 gtaacattgt agaagaagtg actcatccct gtaacccaaa tccttgccct gccaatgagc
     2101 tctgtgaagt aaaccgaaaa ggatgtccat ctggagatcc ctgtcttcca tacttttgtg
     2161 ttcaaggttg caaactggga gaagcttctg atttcattgt ccgtcaaggg acactaatcc
     2221 aggtgccatc atctgcaggg gaagttggtt gttataaaat ctgttcatgt ggacaaagtg
     2281 gactcttaga aaactgtatg gaaatgcact gtatagacct ccagaagtct tgtattgttg
     2341 gaggaaaaag aaaaagtcat ggaacatcct ttagtattga ctgcaatgtc tgttcttgtt
     2401 ttgctggcaa tttggtgtgc tctacccgcc tttgcctcag tgagcacagt tcagaagatg
     2461 accgtcgtac cttcacaggt ctgccctgta actgtgcaga tcagtttgtc cctgtatgtg
     2521 ggcagaatgg gcgcacttac cccagtgcct gcattgctcg ctgtgtgggc ctccaagacc
     2581 atcagtttga gtttggatca tgcatgtcaa aggatccatg taatcctaat ccctgccaaa
     2641 aaaaccaaag gtgcataccc aaaccacagg tctgcctgac gacttttgat aaatttggat
     2701 gtagccagta tgagtgtgta ccaagacagc tcgcgtgtga ccaggtccaa gatcctgttt
     2761 gtgacacaga ccacatggag cacaacaatc tctgcacttt ataccaaaga ggaaaaagcc
     2821 tctcttacaa aggtccctgc cagccctttt gcagagcaac cgagcccgta tgtgggcaca
     2881 atggtgagac ctacagcagt gtgtgtgctg cctactcgga tcgcgtggca gtcgattact
     2941 atggggactg ccaggccgtc ggagtcctct cagagcacag ctccgtcgcc gagtgtgctt
     3001 ctgtcaagtg tccttcgctc ttggcagctg gatgcaaacc catcatccca ccgggtgctt
     3061 gttgcccatt atgtgctggg atgttaagag ttttatttga caaagaaaaa ctggatacta
     3121 ttgctaaggt aacaaataaa aagccaataa cagttctgga aatacttcag aaaatccgca
     3181 tgcacgtgtc tgtcccacag tgtgatgtgt ttggatactt cagcattgaa tcagaaattg
     3241 tgatcctgat cattcccgtc gatcactatc caaaagctct gcagattgaa gcctgcaata
     3301 aagaagcaga gaagattgag tcccttatca actctgacag cccgactttg gcgtcccatg
     3361 tccctctctc tgccctcatc atttcccagg tacaggtctc cagcagtgtg ccatcggccg
     3421 gtgtcagggc caggccttct tgccactccc tcctccttcc cctcagcttg ggccttgcct
     3481 tgcacttgct ctggacatat aactgactgc ccggccggcc aggcaaaaaa gaaaaagtaa
     3541 ggatccgggg ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg
     3601 ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt
     3661 cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac
     3721 gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg
     3781 acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat
     3841 ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga
     3901 gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct
     3961 gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg
     4021 ctccctcgtt gaccgaatca ccgacctctc tccccagcaa ttcaccatgg ccaagccttt
     4081 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     4141 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     4201 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
     4261 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     4321 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
     4381 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     4441 ctctggttat gtgtgggagg gcgaaggtag aggttctctc ctcacttgtg gtgatgttga
     4501 agaaaaccct ggtccaatgt ctagactgga caagagcaaa gtcataaacg gagctctgga
     4561 attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg
     4621 agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct
     4681 gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg
     4741 gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct cacatcgcga
     4801 cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa
     4861 tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc
     4921 cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa
     4981 agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga
     5041 gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg
     5101 tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc ttgacgattt
     5161 tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc
     5221 tgacgctctt gacgattttg accttgacat gctccccggg taactaagta aggatcgatc
     5281 caagatatcg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5341 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5401 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5461 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5521 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5581 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5641 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5701 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5761 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5821 tttgggccgc ctccccgcct gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc
     5881 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5941 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     6001 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6061 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6121 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     6181 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6241 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6301 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6361 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     6421 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6481 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggcctt
     6541 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6601 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6661 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6721 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6781 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6841 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6901 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6961 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7021 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7081 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7141 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7201 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7261 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7321 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7381 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7441 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7501 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7561 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7621 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7681 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7741 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7801 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7861 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7921 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7981 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8041 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8101 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8161 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8221 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8281 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8341 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8401 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     8461 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     8521 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     8581 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     8641 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     8701 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     8761 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     8821 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     8881 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     8941 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9001 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9061 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     9121 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     9181 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     9241 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     9301 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     9361 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     9421 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     9481 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     9541 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     9601 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     9661 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     9721 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     9781 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     9841 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     9901 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     9961 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
    10021 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
    10081 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
    10141 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
    10201 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
    10261 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    10321 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
    10381 cccacctccc aaccccgagg ggac
//
