LOCUS       sequence-494535-        8605 bp    DNA     circular SYN 03-DEC-2025
DEFINITION  Expresses the fluorescent protein mTagBFP2 in a lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Wieland FC, Sthijns MMJPE, Geuens T, van Blitterswijk CA, LaPointe 
            VLS
  TITLE     The Role of Pancreatic Alpha Cells and Endothelial Cells in the 
            Reduction of Oxidative Stress in Pseudoislets.
  JOURNAL   Front Bioeng Biotechnol. 2021 Sep 9;9:729057. doi: 
            10.3389/fbioe.2021.729057. eCollection 2021.
  PUBMED    34568302
REFERENCE   2  (bases 1 to 8605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2021-09-9"; volume: "9:729057. doi"; 
            pages: " 10.3389/fbioe.2021.729057. eCollection 2021"
COMMENT     Sequence Label: pLenti6.2_mTagBFP2
FEATURES             Location/Qualifiers
     source          1..8605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     171..191
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             412..645
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    717..838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(754..773)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     808..827
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      878..1013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1033..1052)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1034..1052)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1062..1079)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1062..1078)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1071..1093)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1220..1675
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1307..1326)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1517..1538
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1701..1805
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1806..1874,1875..2666)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2024..2043)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2837..3425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3326..3345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3579..3596
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3713..3734
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3749..3754,3755..3772,3773..3779)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3787..3803
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3792..3814
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3811..3827
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3811..3827
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3846..3866
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3848..3866
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3894..4120
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4121..4301
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4348..4473
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4966..5199
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5384..5428
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5695..5811
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        5834..6137
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6138..6341
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6292..6312
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6338..6362
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6441..7142
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    7158..7746
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7211..7231)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7629..7640)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7760..8259
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7795..7818)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8192..8211
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(8280..8605,1..274)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8280..8299)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
       61 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
      121 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
      181 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
      241 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
      301 gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta agaccaatga
      361 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
      421 tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
      481 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      541 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      601 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
      661 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
      721 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      781 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
      841 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
      901 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
      961 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     1021 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     1081 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     1141 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     1201 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     1261 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     1321 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     1381 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     1441 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     1501 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     1561 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     1621 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     1681 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1741 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1801 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     1861 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1921 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1981 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2041 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     2101 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     2161 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2221 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2281 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2341 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     2401 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     2461 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     2521 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     2581 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     2641 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     2701 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     2761 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     2821 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2881 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2941 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3001 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3061 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3121 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3181 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3241 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3301 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3361 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3421 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3481 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3541 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3601 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3661 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     3721 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     3781 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     3841 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     3901 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     3961 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     4021 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     4081 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     4141 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4201 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4261 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     4321 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     4381 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     4441 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     4501 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     4561 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     4621 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     4681 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     4741 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     4801 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     4861 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     4921 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     4981 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     5041 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     5101 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     5161 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     5221 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     5281 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     5341 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     5401 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     5461 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     5521 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     5581 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     5641 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     5701 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     5761 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag
     5821 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     5881 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     5941 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     6001 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     6061 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     6121 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     6181 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     6241 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     6301 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     6361 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagagat
     6421 atcttcgaag gcgcgccacc atgagcgagc tgattaagga gaacatgcac atgaagctgt
     6481 acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc
     6541 cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg
     6601 ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc
     6661 agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca
     6721 ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct
     6781 gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc
     6841 agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc
     6901 tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg
     6961 ccaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact
     7021 atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc
     7081 acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatt
     7141 aaagcggccg cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7201 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7261 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7321 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7381 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     7441 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     7501 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     7561 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     7621 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     7681 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     7741 ccccgcctgg aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     7801 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     7861 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     7921 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     7981 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     8041 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     8101 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     8161 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     8221 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
     8281 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     8341 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     8401 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     8461 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     8521 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     8581 cccggctggc cgcgcagcaa cagat
//
