LOCUS       sequence-494567-        8441 bp    DNA     circular SYN 04-DEC-2025
DEFINITION  This plasmid is a modified version of pRG/III-hs-MBP (Addgene 
            plasmid #121676), designed for the expression of a functional 
            beta-1-adrenergic receptor (beta-1AR) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Abiko LA, Rogowski M, Gautier A, Schertler G, Grzesiek S
  TITLE     Efficient production of a functional G protein-coupled receptor in 
            E. coli for structural studies.
  JOURNAL   J Biomol NMR. 2021 Jan;75(1):25-38. doi: 10.1007/s10858-020-00354-6.
            Epub 2021 Jan 27.
  PUBMED    33501610
REFERENCE   2  (bases 1 to 8441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s10858-020-00354-6"; journalName: "J Biomol NMR"; date: 
            "2021-01"; volume: "75"; issue: "1"; pages: "25-38"
COMMENT     Sequence Label: MBP_TS-B1AR_Trx_PreSc_NdeI_AgeI
FEATURES             Location/Qualifiers
     source          1..8441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        187..264
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             265..1347
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALDHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(284..303)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    1360..1381
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1396..1401,1402..1419,1420..1426)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1434..1450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1439..1461
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1458..1474
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1458..1474
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(1510..1587,1588..2685)
                     /codon_start=1
                     /gene="malE"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This gene encodes a signal sequence that can direct 
                     MBP to the bacterial periplasm."
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNG
                     LAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAE
                     ITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDK
                     ELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLV
                     DLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPS
                     KPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKD
                     PRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      2542..2551
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2659..2682
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2716..2739
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3733..3756
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3793..4116
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="SDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEI
                     ADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLD
                     ANLA"
     CDS             4123..4152
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(4174..4191)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4174..4190)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4183..4205)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4369..4386)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4539..4625
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4717..4744
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4763..4854
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4855..4923,4924..5715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5073..5092)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     6783..6802
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      7000..7588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7489..7508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7742..7759
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7925..7947
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7941..8132)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
       61 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
      121 ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga
      181 aattccgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa
      241 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
      301 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
      361 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
      421 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
      481 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
      541 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
      601 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
      661 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
      721 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
      781 catctggtcg cattggatca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
      841 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
      901 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
      961 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
     1021 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
     1081 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat
     1141 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     1201 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     1261 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1321 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1381 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1441 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaatttc tagataacga
     1501 gggcaaacca tgaaaataaa aacaggtgca cgcatcctcg cattatccgc attaacgacg
     1561 atgatgtttt ccgcctcggc tctcgccaaa atcgaagaag gtaaactggt aatctggatt
     1621 aacggcgata aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc
     1681 ggaattaaag tcaccgttga gcatccggat aaactggaag agaaattccc acaggttgcg
     1741 gcaactggcg atggccctga cattatcttc tgggcacacg accgctttgg tggctacgct
     1801 caatctggcc tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg
     1861 tttacctggg atgccgtacg ttacaacggc aagctgattg cttacccgat cgctgttgaa
     1921 gcgttatcgc tgatttataa caaagatctg ctgccgaacc cgccaaaaac ctgggaagag
     1981 atcccggcgc tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg
     2041 caagaaccgt acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat
     2101 gaaaacggca agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt
     2161 ctgaccttcc tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc
     2221 atcgcagaag ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca
     2281 tggtccaaca tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag
     2341 ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc cgccagtccg
     2401 aacaaagagc tggcaaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa
     2461 gcggttaata aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg
     2521 gcgaaagatc cacgtattgc cgccaccatg gaaaacgccc agaaaggtga aatcatgccg
     2581 aacatcccgc agatgtccgc tttctggtat gccgtgcgta ctgcggtgat caacgccgcc
     2641 agcggtcgtc agactgtcga tgaagccctg aaagacgcgc agactggatc tggttctggt
     2701 tctggctcag ggtcactgga agttctgttc caggggcccg gtagcggaag tggatccggg
     2761 agcggacata tgggggccga gctgctgtcg cagcagtggg aggcgggcat gagcctgctg
     2821 atggccctgg tggtgctgct catcgtggcc ggcaacgtgc tggtgatcgc ggccatcggg
     2881 agcacgcagc ggctgcagac gctcaccaac ctcttcatca cctcgctggc ctgcgccgac
     2941 ctggtggtgg ggctgctggt ggtgcctttc ggggccacgc tggtggtgcg gggcacctgg
     3001 ctgtggggct ccttcctctg cgagctctgg acatcgctgg acgtgctttg cgtgacggca
     3061 agcgtggaga ccttgtgcgt catcgccatc gaccgctacc tggccatcac ctctccattc
     3121 cgctaccaga gcctgatgac cagggctcgg gccaaggtca tcatctgcac cgtctgggcc
     3181 atctccgctc tggtctcttt cctgcccatc atgatgcact ggtggcggga cgaggaccct
     3241 caggcgctca agtgctacca ggacccgggc tgctgcgaat ttgtcaccaa ccgggcttac
     3301 gccatcgcct cgtccatcat ctccttctac atccccctcc tcatcatgat cttcgtggcg
     3361 ctgcgggtgt accgggaggc caaggagcag atcaggaaga tcgaccgcgc tagcaagagg
     3421 aagacgtccc gtgtcatgct gatgagggaa cacaaagctc tgaagacatt gggtatcatc
     3481 atgggggtgt tcaccctctg ctggctccct ttcttcttgg tgaacattgt caacgtcttc
     3541 aacagagatc tggtgccgaa atggctcttc gttgccttca actggttggg ctacgccaac
     3601 tctgctatga accccatcat cctctgccgc agcccagact tccgtaaggc cttcaagagg
     3661 ctgctcgcct tcccccgcaa agctgacagg cggctgctcg agggcaccgg tagtggtagt
     3721 ggatctggta gtctggaagt gctgtttcag ggcccgggta gcggtagtgg ctccggctcc
     3781 gggagcgaat tcagcgataa aattattcac ctgactgacg acagttttga cacggatgta
     3841 ctcaaagcgg acggggcgat cctcgtcgat ttctgggcag agtggtgcgg tccgtgcaaa
     3901 atgatcgccc cgattctgga tgaaatcgct gacgaatatc agggcaaact gaccgttgca
     3961 aaactgaaca tcgatcaaaa ccctggcact gcgccgaaat atggcatccg tggtatcccg
     4021 actctgctgc tgttcaaaaa cggtgaagtg gcggcaacca aagtgggtgc actgtctaaa
     4081 ggtcagttga aagagttcct cgacgctaac ctggcgggta cccaccatca ccatcaccat
     4141 caccatcacc attaataatc tagaaagctt ggcactggcc gtcgttttac aacgtcgtga
     4201 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4261 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     4321 tggcgaatgg cagcttggct gttttggcgg atgagataag attttcagcc tgatacagat
     4381 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
     4441 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     4501 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     4561 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     4621 caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag
     4681 gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc
     4741 tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg
     4801 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4861 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4921 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4981 ggttacatcg aactggatct caatagcggt aagatccttg agagttttcg ccccgaagaa
     5041 cgtttcccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     5101 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5161 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5221 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5281 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5341 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5401 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5461 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5521 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5581 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5641 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5701 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaccc
     5761 cggttgataa tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat atttaaattg
     5821 taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     5881 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagccc gagatccggc
     5941 cgaacaaact ccgggaggca gcgtgatgcg gcaacaatca cacggatttc ccgtgaacgg
     6001 tctgaatgag cggattattt tcagggaaag tgagtgtggt cagcgtgcag gtatatgggc
     6061 tatgatgtgc ccggcgcttg aggctttctg cctcatgacg tgaaggtggt ttgttgccgt
     6121 gttgtgtggc agaaagaaga tagccccgta gtaagttaat tttcattaac caccacgagg
     6181 catccctatg tctagtccac atcaggatag cctcttaccg cgctttgcgc aaggagaaga
     6241 aggccatgaa actaccacga agttcccttg tctggtgtgt gttgatcgtg tgtctcacac
     6301 tgttgatatt cacttatctg acacgaaaat cgctgtgcga gattcgttac agagacggac
     6361 acagggaggt ggcggctttc atggcttacg aatccggtaa gtagcaacct ggaggcgggc
     6421 gcaggcccgc cttttcagga ctgatgctgg tctgactact gaagcgcctt tataaagggg
     6481 ctgctggttc gccggtagcc cctttctcct tgctgatgtt gtgggaattc ctgaatcgcc
     6541 ccatcatcca gccagaaagt gagggagcca cggttgatga gagctttgtt gtaggtggac
     6601 cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgt
     6661 gaaccatcac ccaaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     6721 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     6781 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     6841 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgta aaaggatcta
     6901 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6961 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7021 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7081 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7141 tactgtcctt ctagtgtagc ggtagttagg ccaccacttc aagaactctg tagcaccgcc
     7201 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagttgtg
     7261 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7321 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7381 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7441 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7501 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7561 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7621 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7681 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7741 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     7801 tctgtgcggt atttcacacc gcatagggtc atggctgcgc cccgacaccc gccaacaccc
     7861 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7921 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     7981 ctgcggtaaa gctcatcagc gtggtcgtgc agcgattcac agatgtctgc ctgttcatcc
     8041 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     8101 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
     8161 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     8221 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
     8281 cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt
     8341 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
     8401 gctgacttcc gcgtttccag actttacgaa acacggaaac c
//
