LOCUS       sequence-494632-       10186 bp    DNA     circular SYN 04-DEC-2025
DEFINITION  Lentiviral vector that can express FKBP12 along with N terminal 
            split TEV. Expressed off of CMV tet ON promoter with dTomato marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   Chacko AN, Dhanabalan KM, Wan J, Chien R, Anderson NT, Xu B, Pham K,
            Tiwari R, Mukherjee A
  TITLE     A programmable genetic platform for engineering noninvasive 
            biosensors
  JOURNAL   bioRxiv 2025.09.07.674790
REFERENCE   2  (bases 1 to 10186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.07.674790"
COMMENT     Sequence Label: pANC98a(3xFLAG-NES-FKBP12-(G4S)4-nTEV-IRES-dTo...
FEATURES             Location/Qualifiers
     source          1..10186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2976
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2998..3312
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3006..3024
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3042..3060
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3077..3095
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3113..3131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3149..3167
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3184..3202
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3220..3238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3285..3309
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3390..3414
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3443..3495
                     /label=MCS
                     /note="multiple cloning site"
     CDS             3537..3602
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(3654..3656,3657..3977)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     misc_feature    join(4389..4963,4964..4966,4967..4975)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4556..4573)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4783..4802
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4976..5677
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(5109..5127)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5377..5396
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5571..5594
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(5705..5729)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5760..6348
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5813..5833)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6231..6242)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6426..6449)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6788..6807
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6869..7672
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6932..6951)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7542..7561
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             7763..7995
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8067..8188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8104..8123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8158..8177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8228..8363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8383..8402)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8384..8402)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8412..8429)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8412..8428)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8421..8443)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8570..9025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8657..8676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8867..8888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9051..9155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9156..9224,9225..10016)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9374..9393)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaaattcaa aattttatcg atcacgagac tagcctcgag
     3001 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     3061 acgatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     3121 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     3181 ttatccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     3241 gtatgtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac
     3301 cgtcagatcg cctggagaat tcgagctcgg tacccgggga tccaccggtt cgtcgactag
     3361 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggctttaaag
     3421 gaaccaattc agtcgactgg atctcgagct caagcttcga attctgcagt cgacggtacc
     3481 gcgggcccgg gatccaccgg tcgccaccga gctcggatcc agtacccttc accatggact
     3541 acaaagacca cgacggcgat tacaaggatc atgacatcga ttacaaagac gatgacgata
     3601 agatggaaga gctgagccag gctctggcca gctcctttag cgtgtcctcc ggaatgggag
     3661 tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt ccccaagcgc ggccagacct
     3721 gcgtggtgca ctacaccggg atgcttgaag atggaaagaa atttgattcc tcccgggaca
     3781 gaaacaagcc ctttaagttt atgctaggca agcaggaggt gatccgaggc tgggaagaag
     3841 gggttgccca gatgagtgtg ggtcagagag ccaaactgac tatatctcca gattatgcct
     3901 atggtgccac tgggcaccca ggcatcatcc caccacatgc cactctcgtc tttgatgtgg
     3961 agcttctaaa actggaagga tccggtggtg gcgggtctgg aggaggaggt agtggtggag
     4021 gtggctctgg taccgagagc ttgtttaagg ggccgcgtga ttacaacccg atatcgagca
     4081 ccatttgtca tttgacgaat gaatctgatg ggcacacaac atcgttgtat ggtattggat
     4141 ttggtccctt catcattaca aacaagcact tgtttagaag aaataatgga acactgttgg
     4201 tccaatcact acatggtgta ttcaaggtca agaacaccac gactttgcaa caacacctca
     4261 ttgatgggag ggacatgata attattcgca tgcctaagga tttcccacca tttcctcaaa
     4321 agctgaaatt tagagagcca caaagggaag agcgcatatg tcttgtgaca accaacttcc
     4381 aaacttagcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     4441 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     4501 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     4561 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     4621 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     4681 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     4741 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     4801 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     4861 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     4921 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt
     4981 gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc
     5041 catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     5101 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
     5161 gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga
     5221 ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     5281 cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa
     5341 ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat
     5401 gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat
     5461 ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta
     5521 catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat
     5581 cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca
     5641 ccacctgttc ctgtacggca tggacgagct gtacaagtag agcggccgca ctcgagatat
     5701 ctagacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgaca
     5761 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5821 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5881 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5941 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6001 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6061 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6121 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     6181 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6241 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6301 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattctgc
     6361 agatatccgg actagtgatc taattctacc gggtagggga ggcgcttttc ccaaggcagt
     6421 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     6481 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     6541 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     6601 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     6661 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     6721 tcgctttctg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     6781 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     6841 tctccgggcc tttcgacctg cagccaatat gggatcggcc attgaacaag atggattgca
     6901 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     6961 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     7021 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     7081 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     7141 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     7201 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     7261 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     7321 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     7381 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     7441 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     7501 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     7561 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     7621 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaggggatcc
     7681 gtcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7741 tttttaaaag aaaagggggg actggaaggc tagctcactc ccaacgaaga caagatctgc
     7801 tttttgcttg tactgggtct ctctagttag accagatctg agcctgggag ctctctggct
     7861 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7921 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7981 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     8041 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     8101 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8161 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     8221 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8281 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8341 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     8401 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     8461 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     8521 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     8581 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8641 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8701 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8761 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8821 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8881 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8941 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     9001 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9061 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9121 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9181 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9241 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9301 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9361 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9421 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9481 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9541 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9601 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9661 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     9721 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     9781 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     9841 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9901 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9961 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10021 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10081 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10141 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
