LOCUS       sequence-494633-       10835 bp    DNA     circular SYN 04-DEC-2025
DEFINITION  Express ALDH2 with E504K mutation from hSyn1 promoter, co-expressed 
            with EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10835)
  AUTHORS   Sun JK, Wu D, Wong GC, Lau TM, Yang M, Hart RP, Kwan KM, Chan HYE, 
            Chow HM
  TITLE     Chronic alcohol metabolism results in DNA repair infidelity and cell
            cycle-induced senescence in neurons.
  JOURNAL   Aging Cell. 2023 Feb;22(2):e13772. doi: 10.1111/acel.13772. Epub 
            2023 Jan 23.
  PUBMED    36691110
REFERENCE   2  (bases 1 to 10835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Aging 
            Cell."; date: "2023-02"; volume: "22(2):e13772. doi"; pages: " 
            10.1111/acel.13772. Epub 2023 Jan 23"
COMMENT     Sequence Label: FSW-ALDH2-E504K-T2A-EGFP
FEATURES             Location/Qualifiers
     source          1..10835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..448
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             2055..2084
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2097..2150
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2151..2153,2154..2864)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2151..2153,2154..2864)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2193..2214)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2454..2473)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2801..2822
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2905..3493
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2958..2978)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3376..3387)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3496..3512)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3497..3513)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4018..4198
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4224..4241)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4230..4454
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4500..4928
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4587..4606)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4797..4818
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4937..4957)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4942..5271
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5122..5257
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5184..5203
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5319..5366
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5385..5759
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5889..6010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5926..5945)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5980..5999
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6059..6075)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6059..6075)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6072..6094)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6083..6099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6107..6113,6114..6131,6132..6137))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6152..6173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6290..6307)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6461..7049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6541..6560)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7220..8011,8012..8080))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7843..7862
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8081..8185)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8260..8279)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8451..8830
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8832..9030
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8985..9005
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9048..9228
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9275..9400
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9893..10126
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10311..10355
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10653..10770
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
       61 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      121 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      181 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      241 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      301 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      361 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      421 aggagtcgtg tcgtgcctga gagcgcagtg atccggtacc gaggagatct gccgccgcga
      481 tcgccatgtt gcgcgctgcc gcccgcttcg ggccccgcct gggccgccgc ctcttgtcag
      541 ccgccgccac ccaggccgtg cctgccccca accagcagcc cgaggtcttc tgcaaccaga
      601 ttttcataaa caatgaatgg cacgatgccg tcagcaggaa aacattcccc accgtcaatc
      661 cgtccactgg agaggtcatc tgtcaggtag ctgaagggga caaggaagat gtggacaagg
      721 cagtgaaggc cgcccgggcc gccttccagc tgggctcacc ttggcgccgc atggacgcat
      781 cacacagggg ccggctgctg aaccgcctgg ccgatctgat cgagcgggac cggacctacc
      841 tggcggcctt ggagaccctg gacaatggca agccctatgt catctcctac ctggtggatt
      901 tggacatggt cctcaaatgt ctccggtatt atgccggctg ggctgataag taccacggga
      961 aaaccatccc cattgacgga gacttcttca gctacacacg ccatgaacct gtgggggtgt
     1021 gcgggcagat cattccgtgg aatttcccgc tcctgatgca agcatggaag ctgggcccag
     1081 ccttggcaac tggaaacgtg gttgtgatga aggtagctga gcagacaccc ctcaccgccc
     1141 tctatgtggc caacctgatc aaggaggctg gctttccccc tggtgtggtc aacattgtgc
     1201 ctggatttgg ccccacggct ggggccgcca ttgcctccca tgaggatgtg gacaaagtgg
     1261 cattcacagg ctccactgag attggccgcg taatccaggt tgctgctggg agcagcaacc
     1321 tcaagagagt gaccttggag ctggggggga agagccccaa catcatcatg tcagatgccg
     1381 atatggattg ggccgtggaa caggcccact tcgccctgtt cttcaaccag ggccagtgct
     1441 gctgtgccgg ctcccggacc ttcgtgcagg aggacatcta tgatgagttt gtggagcgga
     1501 gcgttgcccg ggccaagtct cgggtggtcg ggaacccctt tgatagcaag accgagcagg
     1561 ggccgcaggt ggatgaaact cagtttaaga agatcctcgg ctacatcaac acggggaagc
     1621 aagagggggc gaagctgctg tgtggtgggg gcattgctgc tgaccgtggt tacttcatcc
     1681 agcccactgt gtttggagat gtgcaggatg gcatgaccat cgccaaggag gagatcttcg
     1741 ggccagtgat gcagatcctg aagttcaaga ccatagagga ggttgttggg agagccaaca
     1801 attccacgta cgggctggcc gcagctgtct tcacaaagga tttggacaag gccaattacc
     1861 tgtcccaggc cctccaggcg ggcactgtgt gggtcaactg ctatgatgtg tttggagccc
     1921 agtcaccctt tggtggctac aagatgtcgg ggagtggccg ggagttgggc gagtacgggc
     1981 tgcaggcata cactaaagtg aaaactgtca cagtcaaagt gcctcagaag aactcaacgc
     2041 gtacgcggcc gctcgagcag aaactcatct cagaagagga tctggcagca aatgatgagg
     2101 gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca gtgagcaagg
     2161 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2221 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2281 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2341 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     2401 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2461 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2521 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2581 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2641 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2701 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     2761 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2821 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caaggaattc taactagagc
     2881 tcgctcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     2941 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3001 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3061 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3121 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3181 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3241 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3301 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3361 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3421 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3481 gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca
     3541 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     3601 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     3661 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     3721 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     3781 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     3841 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     3901 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     3961 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     4021 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     4081 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     4141 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     4201 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4261 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4321 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4381 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4441 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4501 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4561 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4621 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4681 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4741 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4801 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4861 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4921 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4981 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     5041 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5101 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5161 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     5221 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     5281 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     5341 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     5401 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     5461 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     5521 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     5581 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     5641 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     5701 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     5761 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5821 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     5881 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5941 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6001 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     6061 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6121 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6181 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6241 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6301 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6361 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6421 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6481 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6541 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6601 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6661 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6721 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6781 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6841 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6901 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6961 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7021 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7081 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7141 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7201 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7261 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7321 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7381 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7441 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7501 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7561 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7621 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7681 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7741 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7801 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7861 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7921 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7981 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8041 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8101 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8161 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     8221 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     8281 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     8341 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     8401 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     8461 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     8521 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     8581 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     8641 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     8701 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     8761 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     8821 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     8881 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     8941 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     9001 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
     9061 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9121 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9181 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     9241 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     9301 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     9361 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     9421 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     9481 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     9541 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     9601 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     9661 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     9721 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     9781 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     9841 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     9901 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     9961 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
    10021 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
    10081 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
    10141 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
    10201 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
    10261 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
    10321 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
    10381 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
    10441 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
    10501 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
    10561 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
    10621 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
    10681 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
    10741 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
    10801 gtttggttaa ttaactgcag agggccctgc gtatg
//
