LOCUS       sequence-494651-       10051 bp    DNA     circular SYN 04-DEC-2025
DEFINITION  Lentiviral vector that can express TVMVp. Expressed off of CMV tet 
            ON promoter with dTomato marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Chacko AN, Dhanabalan KM, Wan J, Chien R, Anderson NT, Xu B, Pham K,
            Tiwari R, Mukherjee A
  TITLE     A programmable genetic platform for engineering noninvasive 
            biosensors
  JOURNAL   bioRxiv 2025.09.07.674790
REFERENCE   2  (bases 1 to 10051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.07.674790"
COMMENT     Sequence Label: pANC76(TVMVp-IRES-dTomato)
FEATURES             Location/Qualifiers
     source          1..10051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(93..112)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(94..112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(122..139)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(122..138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(131..153)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      280..735
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(367..386)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     577..598
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        761..865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(866..934,935..1726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1084..1103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1897..2485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2386..2405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2639..2656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2773..2794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2809..2814,2815..2832,2833..2839)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2847..2863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2852..2874
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2871..2887
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2871..2887
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2906..2926
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2908..2926
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2954..3180
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3181..3361
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3408..3533
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4026..4259
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4444..4488
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4755..4872
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4894..5208
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4902..4920
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4938..4956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4973..4991
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5009..5027
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5045..5063
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5080..5098
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5116..5134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5181..5205
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5286..5310
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5339..5391
                     /label=MCS
                     /note="multiple cloning site"
     CDS             5439..6149
                     /codon_start=1
                     /product="tobacco vein mottling virus NIa protease 
                     (Nallamsetty et al., 2004)"
                     /label=TVMV protease
                     /translation="SKALLKGVRDFNPISACVCLLENSSDGHSERLFGIGFGPYIIANQ
                     HLFRRNNGELTIKTMHGEFKVKNSTQLQMKPVEGRDIIVIKMAKDFPPFPQKLKFRQPT
                     IKDRVCMVSTNFQQKSVSSLVSESSHIVHKEDTSFWQHWITTKDGQCGSPLVSIIDGNI
                     LGIHSLTHTTNGSNYFVEFPEKFVATYLDAADGWCKNWKFNADKISWGSFTLVEDAPED
                     DFMAKKTVAAIMDS"
     misc_feature    join(6150..6724,6725..6727,6728..6736)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6317..6334)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6544..6563
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6737..7438
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(6870..6888)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7138..7157
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7332..7355
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(7466..7490)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7521..8109
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7574..7594)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7992..8003)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8187..8210)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8549..8568
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8630..9433
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8693..8712)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9303..9322
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             9524..9756
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9828..9949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9865..9884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9919..9938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      join(9989..10051,1..73)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
       61 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
      121 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      181 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      241 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
      301 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      361 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      421 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      481 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      541 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      601 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
      661 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
      721 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      781 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      841 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      901 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      961 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1021 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1081 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1141 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1201 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1261 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1321 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1381 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1441 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1501 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1561 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1621 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1681 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1741 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1801 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1861 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1921 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1981 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2041 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2101 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2161 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2221 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2281 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2341 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2401 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2461 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2521 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2581 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2641 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2701 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2761 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     2821 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     2881 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     2941 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     3001 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     3061 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     3121 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     3181 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3241 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3301 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3361 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     3421 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     3481 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     3541 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     3601 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     3661 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     3721 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     3781 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     3841 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     3901 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3961 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     4021 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     4081 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     4141 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     4201 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     4261 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     4321 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     4381 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     4441 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     4501 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     4561 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     4621 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     4681 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     4741 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     4801 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     4861 attcaaaatt ttatcgatca cgagactagc ctcgagttta ctccctatca gtgatagaga
     4921 acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc
     4981 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     5041 ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac
     5101 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg
     5161 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattcga
     5221 gctcggtacc cggggatcca ccggttcgtc gactagtcca gtgtggtgga attctgcaga
     5281 tatcaacaag tttgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatct
     5341 cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc
     5401 caccgagctc ggatccagta cccttcacca tgggatcctc aaaagcactc ctgaagggag
     5461 tccgcgactt taatcctata tcagcgtgtg tatgtttgct tgaaaactcc tctgatgggc
     5521 attcagaaag gctttttggt attgggtttg gtccctacat aatagcaaac caacacctgt
     5581 ttcgacgcaa taacggcgag cttacgatta agacgatgca cggggaattt aaagtgaaaa
     5641 acagcacaca attgcaaatg aaacccgtcg aagggagaga catcattgta ataaagatgg
     5701 caaaggattt cccacccttt ccgcagaaat tgaaattccg gcaacctact ataaaggatc
     5761 gagtttgtat ggtttctacg aactttcagc aaaaatcagt ctcctccctc gtgtccgaat
     5821 cctctcacat cgttcacaag gaagatacca gtttctggca gcattggatt accacaaaag
     5881 acgggcagtg cggctcaccg ctggtgtcta tcattgacgg gaacattctg ggaatccact
     5941 ccctgacaca caccactaac ggctcaaact atttcgttga gttcccagag aagttcgtag
     6001 ctacatatct ggacgctgcg gacggctggt gcaagaactg gaaattcaac gcggacaaga
     6061 tttcatgggg tagtttcacg cttgtcgaag acgcgcccga ggacgacttt atggcgaaaa
     6121 aaaccgtggc tgctattatg gatagctagc ccctctccct cccccccccc taacgttact
     6181 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     6241 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     6301 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     6361 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     6421 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     6481 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     6541 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     6601 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     6661 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     6721 taatatggcc acaaccatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt
     6781 caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
     6841 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
     6901 gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa
     6961 gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga
     7021 gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca
     7081 ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc
     7141 cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga
     7201 cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct
     7261 ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta
     7321 cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     7381 cgagcgctcc gagggccgcc accacctgtt cctgtacggc atggacgagc tgtacaagta
     7441 gagcggccgc actcgagata tctagaccca gctttcttgt acaaagtggt tgatatccag
     7501 cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     7561 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     7621 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7681 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7741 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7801 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7861 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     7921 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7981 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     8041 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     8101 cctccccgcc tggaattctg cagatatccg gactagtgat ctaattctac cgggtagggg
     8161 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
     8221 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     8281 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     8341 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     8401 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     8461 gccaatagca gctttgctcc ttcgctttct gggctcaggg gcgggctcag gggcggggcg
     8521 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     8581 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagccaata tgggatcggc
     8641 cattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     8701 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     8761 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     8821 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     8881 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     8941 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     9001 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     9061 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     9121 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     9181 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     9241 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     9301 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     9361 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     9421 cgagttcttc tgaggggatc cgtcgagggc ccggtacctt taagaccaat gacttacaag
     9481 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg ctagctcact
     9541 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctagtta gaccagatct
     9601 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9661 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9721 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     9781 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     9841 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9901 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     9961 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
    10021 ttctccgccc catggctgac taattttttt t
//
