LOCUS       sequence-494665-       10030 bp    DNA     circular SYN 04-DEC-2025
DEFINITION  Express Arl13B (resistance to sgRNA), tagged with GFP, with R200C 
            mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Mahajan D, Chia HM, Lu L
  TITLE     Knocking out ARL13B completely abolishes primary ciliogenesis in 
            cell lines.
  JOURNAL   Sci Rep. 2025 Jul 24;15(1):26980. doi: 10.1038/s41598-025-11124-5.
  PUBMED    40707593
REFERENCE   2  (bases 1 to 10030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2025-07-24"; volume: "15(1):26980. doi"; pages: " 
            10.1038/s41598-025-11124-5"
COMMENT     Sequence Label: ARL13B-(R200C)-GFP
FEATURES             Location/Qualifiers
     source          1..10030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2200..2503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2504..2707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2658..2678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2704..2728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(4131..4133,4134..4136,4137..4847)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4131..4133,4134..4136,4137..4847)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4176..4197)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4437..4456)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4784..4805
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4864..5363
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4899..4922)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5296..5315
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5384..5983
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5384..5403)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5880..5900
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5997..6585
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6050..6070)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6468..6479)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6792..7425
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7554..7570)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7554..7570)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7567..7589)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7578..7594
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7602..7608,7609..7626,7627..7632))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7647..7668
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7785..7802)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7956..8544)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8036..8055)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8715..9506,9507..9575))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9338..9357
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9576..9680)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9728..9862
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9765..9784)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9819..9838
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10030,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     2221 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2281 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2341 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2401 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2461 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2521 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2581 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2641 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2701 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2761 ccatagaaga caccgactct actagaggat cgctagcgct accggactca gatctcgagg
     2821 ccaccatgtt cagtctcatg gcaagctgtt gtggatggtt taaacgctgg cgggagcctg
     2881 ttagaaaggt gactcttttg atggtgggac ttgataatgc tggtaaaacc gcaacagcaa
     2941 agggaatcca aggagaatac cctgaggacg tggcgccaac cgtcggattt tcaaaaatta
     3001 accttagaca gggaaagttt gaagtcacca tctttgactt gggaggtgga ataagaattc
     3061 ggggaatctg gaagaattac tatgctgaat cctatggggt aatatttgtt gtggattcca
     3121 gtgatgaaga gagaatggaa gagacaaaag aggctatgtc agaaatgcta agacatcctc
     3181 gtatcagtgg gaaacccata ttggtgttgg caaataaaca agataaagaa ggagctttag
     3241 gagaagctga tgtcattgaa tgtctatctc tggaaaaatt ggtcaatgag cacaagtgcc
     3301 tgtgtcagat agaaccatgc tccgccattt ccggctatgg aaagaaaatt gacaagtcca
     3361 ttaaaaaagg cctttattgg ctgctacatg ttattgcaag agactttgat gccttaaatg
     3421 aatgcatcca aaaagagaca acagagcagc gtgctcttga ggaacaagag aaacaagaaa
     3481 gagctgaacg agtgcgaaaa ttacgagaag aaagaaaaca aaatgaacag gagcaggctg
     3541 aactcgatgg aaccagtggt ctggctgagt tggacccaga accaacgaat cctttccagc
     3601 caatagcatc tgtaatcatt gagaacgaag gaaaacttga aagagagaaa aaaaaccaaa
     3661 aaatggagaa agacagtgat ggctgccacc tgaaacataa aatggagcat gagcaaatag
     3721 agacacaagg ccaggttaat cacaatggcc aaaaaaataa tgaatttgga ctagtagaaa
     3781 attataagga ggcattaaca cagcagttaa agaatgaaga tgagacagac cggccatcat
     3841 tggaatcagc taatggtaaa aagaaaacta agaaactaag aatgaaaagg aaccaccggg
     3901 tagaaccact taatatagat gactgtgctc ctgagagtcc aacgccaccc ccaccccctc
     3961 ctcctgttgg ctggggaacc cctaaagtca ctagacttcc aaaacttgag cctcttggtg
     4021 aaacacatca taatgatttc tataggaagc cactgcctcc cctggctgtg ccacagcgac
     4081 ctaacagtga tgctcatgat gtgatctcag cggccgctga acgcggatcc atggtgagca
     4141 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     4201 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     4261 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     4321 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     4381 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     4441 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     4501 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4561 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     4621 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     4681 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     4741 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4801 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtct agataattct
     4861 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     4921 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     4981 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     5041 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     5101 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     5161 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     5221 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     5281 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     5341 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
     5401 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     5461 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     5521 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     5581 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     5641 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     5701 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     5761 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     5821 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     5881 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     5941 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc
     6001 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6061 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6121 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6181 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6241 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6301 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6361 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     6421 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6481 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     6541 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg
     6601 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     6661 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     6721 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6781 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     6841 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     6901 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     6961 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     7021 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     7081 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     7141 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     7201 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     7261 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7321 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7381 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     7441 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     7501 gacattgcta gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     7561 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7621 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7681 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7741 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7801 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7861 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7921 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7981 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8041 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8101 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8161 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8221 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8281 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8341 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8401 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8461 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8521 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8581 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8641 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8701 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8761 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8821 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8881 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8941 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9001 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9061 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9121 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9181 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9241 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9301 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9361 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9421 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9481 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9541 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9601 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9661 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     9721 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     9781 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9841 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     9901 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     9961 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    10021 cttagtagtt
//
