LOCUS       sequence-49771-e         821 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Martinez-Noel G, Galligan JT, Sowa ME, Arndt V, Overton TM, Harper 
            JW, Howley PM
  TITLE     Identification and proteomic analysis of distinct UBE3A/E6AP protein
            complexes.
  JOURNAL   Mol Cell Biol. 2012 May 29.
  PUBMED    22645313
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2012 May 29."
COMMENT     Sequence Label: p6899 PHAGE-P CMVT N-HA-hE6AP II  C843A
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    40..58
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     68..92
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      179..188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             191..217
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    233..257
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgggggttta gcagagctct cctatcagtg atagagatct ccctatcagt gatagagatc
       61 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      121 acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacgctagc
      181 caccatggcg tacccatacg atgttccaga ttacgctgct cgaggttatc aaacaagttt
      241 gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg gagaagctgc accagtgtta
      301 ttggaaatca ggagaacctc agtctgacga cattgaagct agccgaatga agcgagcagc
      361 tgcaaagcat ctaatagaac gctactacca ccagttaact gagggctgtg gaaatgaagc
      421 ctgcacgaat gagttttgtg cttcctgtcc aacttttctt cgtatggata ataatgcagc
      481 agctattaaa gccctcgagc tttataagat taatgcaaaa ctctgtgatc ctcatccctc
      541 caagaaagga gcaagctcag cttaccttga gaactcgaaa ggtgccccca acaactcctg
      601 ctctgagata aaaatgaaca agaaaggcgc tagaattgat tttaaagatg tgacttactt
      661 aacagaagag aaggtatatg aaattcttga attatgtaga gaaagagagg attattcccc
      721 tttaatccgt gttattggaa gagttttttc tagtgctgag gcattggtac agagcttccg
      781 gaaagttaaa caacacacca aggaagaact gaaatctctt c
//
