LOCUS       sequence-49784-e         745 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Martinez-Noel G, Galligan JT, Sowa ME, Arndt V, Overton TM, Harper 
            JW, Howley PM
  TITLE     Identification and proteomic analysis of distinct UBE3A/E6AP protein
            complexes.
  JOURNAL   Mol Cell Biol. 2012 May 29.
  PUBMED    22645313
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2012 May 29."
COMMENT     Sequence Label: p6897 PHAGE-P CMVT N-HA-hE6AP III wt
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    35..53
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     63..87
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      174..183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             186..212
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    228..252
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctttagcga gctctcctat cagtgataga gatctcccta tcagtgatag agatcgtcga
       61 cgagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      121 catagaagac accgggaccg atccagcctc cggactctag cgtttaaacg ctagccacca
      181 tggcgtaccc atacgatgtt ccagattacg ctgctcgagg ttatcaaaca agtttgtaca
      241 aaaaagcagg ctccgcggcc gcccccttca ccatggccac agcttgtaaa agatcaggag
      301 aacctcagtc tgacgacatt gaagctagcc gaatgaagcg agcagctgca aagcatctaa
      361 tagaacgcta ctaccaccag ttaactgagg gctgtggaaa tgaagcctgc acgaatgagt
      421 tttgtgcttc ctgtccaact tttcttcgta tggataataa tgcagcagct attaaagccc
      481 tcgagcttta taagattaat gcaaaactct gtgatcctca tccctccaag aaaggagcaa
      541 gctcagctta ccttgagaac tcgaaaggtg cccccaacaa ctcctgctct gagataaaaa
      601 tgaacaagaa aggcgctaga attgatttta aagatgtgac ttacttaaca gaagagaagg
      661 tatatgaaat tcttgaatta tgtagagaaa gagaggatta ttccccttta atccgtgtta
      721 tgtggaagag ttttttctag tgctg
//
