LOCUS       sequence-50295-e         809 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DUSP13
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    691..715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(715..734)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(716..734)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcttgtcgac ggagctcgaa ttcggatcct caatggtggt gatgatggtg cgcgaaccgc
       61 cccgtctccc gccccagtcg gttgtccaga acctggagct gccggaggaa gcctgagtta
      121 gggcagatat tgcggtgggc ctgcaccgtc tggatggcct ctaccagcgt catgttctca
      181 tagatcatga ggaaggccag gacaagtgtg gcagagcggc ttacccccat ggcacagtgt
      241 accagcacgc ggccttgggg aacactgagg gcagctcgga tgtatcgagc aacaggcaga
      301 aagtagacac tgaggtcgaa gaaggggttg tcatccgcct cgatgccata gtactccagg
      361 gacattccac ggtagaattt ggcacctgtg tccacctgga acttgcctgc agcggcattc
      421 acaacgtggg tgattcccag ctggatcagc ttgctcttgt cccgggctgc gtacgcatct
      481 cccaggaaga ggctgggcca gacctcatcg atatggttca gtgtggcagc ctgacggacc
      541 cacagcaagc gctgcagcga agccaatgtg ggcggctggt agggagatgc ctggactgcc
      601 ccatggatct tgggcctccg gaggtcctgc ttctgcagtg agtccatagt atatctcctt
      661 cttaaggtta aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat
      721 agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg
      781 gccgggcatc accggcgcca caggtgcgg
//
