LOCUS       sequence-50399-e         840 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Beier KT, Samson ME, Matsuda T, Cepko CL
  TITLE     Conditional expression of the TVA receptor allows clonal analysis of
            descendents from Cre-expressing progenitor cells.
  JOURNAL   Dev Biol. 2011 May 15;353(2):309-20. Epub 2011 Mar 21.
  PUBMED    21397594
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2011-05-15"; volume: "353(2)"; pages: "309-20. Epub 2011 Mar 
            21"
COMMENT     Sequence Label: pQC membrane Venus IX
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(205..207,208..210,211..723)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(250..271)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(511..530)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acatcagctg ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg
       61 aacggtgcat tggaacggac ctgcaggaat tgatccgcgg ccgcaccggt aggcctcgta
      121 cgcttaatta acggatccgc cgccatgctg tgctgtatga gaagaaccaa acaggttgaa
      181 aagaatgatg aggaccaaaa gatcatggtg agcaagggcg aggagctgtt caccggggtg
      241 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      301 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttaatctg caccaccggc
      361 aagctgcccg tgccctggcc caccctcgtg accaccttag gctacggcct gcagtgcttc
      421 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      481 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      541 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      601 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      661 atcacggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc
      721 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      781 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagactca
//
