LOCUS       sequence-50492-e         925 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GLRX2
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..103
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             499..516
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(569..587)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      583..630
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(754..773)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgaatattt tgtttacttt aagaaggaga tatacatatg caccatcatc atcatcattc
       61 ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgcctg tgaaccagat
      121 ccaagaaaca atttctgata attgtgtggt gattttctca aaaacatcct gttcttactg
      181 tacaatggca aaaaagcttt tccatgacat gaatgttaac tataaagtgg tggaactgga
      241 cctgcttgaa tatggaaacc agttccaaga tgctctttac aaaatgactg gtgaaagaac
      301 tgttccaaga atatttgtca atggtacttt tattggaggt gcaactgaca ctcataggct
      361 tcacaaagaa ggaaaattgc tcccactagt tcatcagtgt tatttaaaaa aaagtaagag
      421 gaaagaattt cagtgacagt aaaggtggat acggatccga attcgagctc cgtcgacaag
      481 cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
      541 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      601 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
      661 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      721 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      781 tcgccacgtt cgccggcttt cccccgtcaa gctctaaatc gggggctccc tttagggttc
      841 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcccgt
      901 agtgggccat cgccctgata gacgg
//
