LOCUS       sequence-50554-e         888 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GPX5
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(694..714)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(739..756)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             767..789
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    804..828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(828..847)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(829..847)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctactcttcg gcttgtagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg
       61 ccgcaagctt gtcgacggag ctcgaattcg gatccgtatc cacctttact gtcacgtttt
      121 aaactgtttc aaataagcca gaatgtcggt cttcacggat gatactgttg ctcgatgact
      181 ccatctcata acgggtatac catccgggcc gactaagaat ttctcaaagt tccaccggat
      241 atcgtgcact ttaacagggt cccaagaaat gctcttaaaa gttccgagga tttccgatgg
      301 atgcgggcag gagtgtttca ggaatgaaaa tactttctgc tccttttccc cattcacatc
      361 acctttttcg aaaagttgaa aactggggac gaagccaccg cccgggcgaa cgtatttcaa
      421 tccaggcaga atctccttgt tgtcccctgg ttcctgttta ccaaattgat tacacggaaa
      481 gcctaataca accagtccat agggtttgag ctcttcctga agggcattca attcagggta
      541 ttgcgccgtt aacccgcaat aggtagccac gttgacgaac agtatatgct tacccacgta
      601 ctgtttgaaa gatacatact cgtttttatt cagtgcgatg gcttcgtaat cataaattgt
      661 gcccttctcg tctttgtgac aatccatttt catggattgg aagtacaggt tctcggtacc
      721 cagatctaca ccagaagaat gatgatgatg atggtgcata tgtatatctc cttcttaaag
      781 ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt
      841 cgtattaatt tcgcgggatc gagactcgat ctctaccgga gacggccg
//
