LOCUS       sequence-50748-e         904 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     2-series LIC periplasmic secretion vectors for E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET PPL His6 LIC cloning vector (2J-T)
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             65..87
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             182..199
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             200..220
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(345..363)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      359..406
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(772..777,778..794,795..800))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(827..845)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtgcggctcc ggagagctct ttaattaagc ggccgccctg caggactcga gttctagaaa
       61 taattttgtt taactttaag aaggagatat acatatgaaa ataaaaacag gtgcacgcat
      121 cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaatcttc
      181 tcaccatcac catcaccatg aaaacctgta cttccaatcc aatattggaa gtggataacg
      241 gatccgcgat cgcggcgcgc cacctggtgg ccggccggta ccacgcgtgc gcgctgatcc
      301 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      361 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      421 tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc
      481 caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa
      541 cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg
      601 cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc
      661 ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt
      721 tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct
      781 tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc
      841 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
      901 cggg
//
