LOCUS       sequence-50789-e         776 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral expression of HPV 6bE6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pLentiN 6bE6
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             109..132
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             145..171
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(653..677)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             730..771
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tagaagacac cgactctaga ggatccacta gtccagtgtg gtggaattct gcagatatca
       61 acaagtttgt acaaaaaagc aggctccgcg gccgccccct tcaccatgga ctacaaggat
      121 gacgatgaca agctcgatgg aggataccca tacgatgttc cagattacgc tgattcgact
      181 ggaatggaaa gtgcaaatgc ctccacgtct gcaacgacca tagaccagtt gtgcaagacg
      241 tttaatctat ctatgcatac gttgcaaatt aattgtgtgt tttgcaagaa tgcactgacc
      301 acagcagaga tttattcata tgcatataaa cacctaaagg tcctgtttcg aggcggctat
      361 ccatatgcag cctgcgcgtg ctgcctagaa tttcatggaa aaataaacca atatagacac
      421 tttgattatg ctggatatgc aacaacagtt gaagaagaaa ctaaacaaga catcttagac
      481 gtgctaattc ggtgctacct gtgtcacaaa ccgctgtgtg aagtagaaaa ggtaaaacat
      541 atactaacca aggcgcggtt cataaagcta aattgtacgt ggaagggtcg ctgcctacac
      601 tgctggacaa catgcatgga agacatgtta ccctaaaagg gtgggcgcgc cgacccagct
      661 ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg
      721 cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtac
//
