LOCUS       sequence-50958-e         812 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Martinez-Noel G, Galligan JT, Sowa ME, Arndt V, Overton TM, Harper 
            JW, Howley PM
  TITLE     Identification and proteomic analysis of distinct UBE3A/E6AP protein
            complexes.
  JOURNAL   Mol Cell Biol. 2012 May 29.
  PUBMED    22645313
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2012 May 29."
COMMENT     Sequence Label: p6900 PHAGE-P CMVT N-HA-hE6AP III C840A
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..45
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     55..79
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      166..175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             178..204
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    220..244
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctctcct atcagtgata gagatctccc tatcagtgat agagatcgtc gacgagctcg
       61 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      121 acaccgggac cgatccagcc tccggactct agcgtttaaa cgctagccac catggcgtac
      181 ccatacgatg ttccagatta cgctgctcga ggttatcaaa caagtttgta caaaaaagca
      241 ggctccgcgg ccgccccctt caccatggcc acagcttgta aaagatcagg agaacctcag
      301 tctgacgaca ttgaagctag ccgaatgaag cgagcagctg caaagcatct aatagaacgc
      361 tactaccacc agttaactga gggctgtgga aatgaagcct gcacgaatga gttttgtgct
      421 tcctgtccaa cttttcttcg tatggataat aatgcagcag ctattaaagc cctcgagctt
      481 tataagatta atgcaaaact ctgtgatcct catccctcca agaaaggagc aagctcagct
      541 taccttgaga actcgaaagg tgcccccaac aactcctgct ctgagataaa aatgaacaag
      601 aaaggcgcta gaattgattt taaagatgtg acttacttaa cagaagagaa ggtatatgaa
      661 attcttgaat tatgtagaga aagagaggat tattcccctt taatccgtgt tattggaaga
      721 gttttttcta gtgctgaggc attggtacag agcttccgga aagttaaaca acacaccaag
      781 ggaagaactg aaatctcttc aagcaaaaga tg
//
