LOCUS       sequence-51242-e         844 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GPX3
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             579..599
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             600..617
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             618..641
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             691..708
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(761..779)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      775..822
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gccatggtgg cataagtggc accatttacg agtacggagc cctcaccatt gatggggagg
       61 agtacatccc cttcaagcag tatgctggca aatacgtcct ctttgtcaac gtggccagct
      121 acggaggcct gacgggccag tacattgaac tgaatgcact acaggaagag cttgcaccat
      181 tcggtctggt cattctgggc tttccctgca accaatttgg aaaacaggaa ccaggagaga
      241 actcagagat ccttcctacc ctcaagtatg tccgaccagg tggaggcttt gtccctaatt
      301 tccagctctt tgagaaaggg gatgtcaatg gagagaaaga gcagaaattc tacactttcc
      361 taaagaactc ctgtcctccc acctcggagc tcctgggtac atctgaccgc ctcttctggg
      421 aacccatgaa ggttcacgac atccgctgga actttgagaa gttcctggtg gggccagatg
      481 gtatacccat catgcgctgg caccaccgga ccacggtcag caacgtcaag atggacatcc
      541 tgtcctacat gaggcggcag gcagccctgg gggtcgcaga gaacctctac ttccaatcgc
      601 accatcatca ccaccatgat tacaaggatg acgacgataa gtgaggatcc gaattcgagc
      661 tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      721 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      781 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      841 tccg
//
