LOCUS       sequence-51592-e         938 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Bahler J, Wu JQ, Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, 
            Wach A, Philippsen P, Pringle JR
  TITLE     Heterologous modules for efficient and versatile PCR-based gene 
            targeting in Schizosaccharomyces pombe.
  JOURNAL   Yeast. 1998 Jul;14(10):943-51.
  PUBMED    9717240
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "943-51"
COMMENT     Sequence Label: pFA6a-kanMX6-P41nmt-3HA
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             137..226
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      259..446
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(314..338)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        complement(522..540)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(523..540)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(621..640)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     740..762
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(800..818)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atcgaaacat tgaagataaa ggaagaggaa tcctggcata tcatcaattg aataagttga
       61 attaattatt tcaatctcat tctcactttc tgacttatag tcgctttgtt aaatcatgtc
      121 tttaattaac atcttttacc catacgatgt tcctgactat gcgggctatc cctatgacgt
      181 cccggactat gcaggatcct atccatatga cgttccagat tacgctgctc agtgctgagg
      241 cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt
      301 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
      361 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc
      421 gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt aatatagatc
      481 cgtcgacctg cagcgtacga agcttcagct ggcggccgcg ttctatagtg tcacctaaat
      541 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
      601 tccatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
      661 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      721 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
      781 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      841 ataatggttt cttagacgtc aggtggcact tttcggggat gtgcgcggaa cccctatttg
      901 tttatttttc taaatacatt caaatatgta tccgctca
//
