LOCUS       sequence-51638-e         713 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00509
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(516..615)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(631..648)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(631..647)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(640..662)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
       61 ctttcttata atgccaactt tgtacaagaa agttggcaag tgaaagcact tggcctttga
      121 gaagcacgtc ctgcagatcg gagccattgc ttcatccttg ctgtcgtaca caaaaagggt
      181 tggcctttgg ttagtttttg agaatcgaga ggacaacgga gcttcataga tatggggaaa
      241 cgactgcgaa aggcagacct ccgtctggtg ctccttgaag gcattccact tctccaactt
      301 ctccttgtac tcggccattg ccttgctggc cgactcctgc cacacgatct tgtgctcatc
      361 ggccatggct cgccacatct caccgccctt cacagacact tcttggacac taaaatcggg
      421 atgctccgct cttatgttct tgcgcccggt ggaattcatc cacaacatga aggcgctcat
      481 gggttttttg gggcgtgctc cgtagtctga catggtgcca acttttttgt acaaagttgg
      541 cattataaaa aagcattgct catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
      601 taaaatcatt atttggggcc cgagcttaag actggccgtc gttttacaac gtcgtgactg
      661 ggaaaacatc catgctagcg ttaacgcgag agtagggaac tgccaggcat caa
//
