LOCUS       sequence-51662-e         914 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Su PC, Berger BW
  TITLE     Identifying Key Juxtamembrane Interactions in Cell Membranes Using 
            AraTM.
  JOURNAL   J Biol Chem. 2012 Jul 22.
  PUBMED    22822084
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Jul 22."
COMMENT     Sequence Label: pAraGFP
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             110..823
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(140..168)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     763..783
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(887..904)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 ctgacgcttt tatcgcactc tctactgttt ctccataccc gtttttttgg gctagcgaat
       61 tcgagctcgg tacccgggga tcctctagat ttaagaagga gatatacata tgagtaaagg
      121 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      181 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      241 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      301 cgcgtatggt cttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
      361 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      421 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      481 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      541 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
      601 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
      661 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
      721 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
      781 aacagctgct gggattacac atggcatgga tgaactatac aaataaatgt ccagacctgc
      841 aggcatgcaa gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta
      901 aatcagaacg caga
//
