LOCUS       sequence-51664-e        8177 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min 
            H, Golden SS
  TITLE     Application of bioluminescence to the study of circadian rhythms in 
            cyanobacteria.
  JOURNAL   Methods Enzymol. 2000;305:527-42.
  PUBMED    10812624
REFERENCE   2  (bases 1 to 8177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2000"; volume: "305"; pages: "527-42"
COMMENT     Sequence Label: pAM1414
FEATURES             Location/Qualifiers
     source          1..8177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4170..4758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4659..4678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4912..4929
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4944..5084
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5010..5029)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5170..5192
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             7003..7794
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ggccgcggat cccctcgact ttatcgagcc tgattttgaa caactcacca tcgcgactgt
       61 gaatgaacgt cgcttgaaag cggaaattga aagccgtacg ccagaaatgg cttaggtctt
      121 atcgtaatac caacaaataa ggaaatgtta tgaaatttgg aaacttcctt ctcacttatc
      181 agccacctga gctatctcag accgaagtga tgaagcgatt ggttaatctg ggcaaagcgt
      241 ctgaaggttg tggcttcgac accgtttggt tgctagagca ccacttcact gaatttgggt
      301 tgttagggaa tccttatgtt gctgccgcac acctattagg tgcgacagaa acgctcaacg
      361 ttggcactgc agctatcgta ttgccgactg cccatccggt tcgacaagca gaagacgtaa
      421 acctactgga tcaaatgtca aaaggacgat tccgttttgg tatttgtcgc ggtttgtacg
      481 ataaagattt tcgtgtcttt ggtacagaca tggataacag ccgagcctta atggactgtt
      541 ggtatgactt gatgaaagaa ggcttcaatg aaggctatat cgcggcggat aacgaacata
      601 ttaagttccc gaaaatccaa ctgaatccat cggcttacac acaaggtggt gctcctgttt
      661 atgtcgtcgc ggagtcagca tcaacgacag aatgggctgc agagcgtggc ctaccaatga
      721 ttctaagctg gatcatcaac actcacgaga agaaagcgca gcttgatctt tacaacgaag
      781 tcgcgactga acatggctac gatgtgacta agattgacca ctgtttgtct tacatcacct
      841 ccgtcgatca tgactcaaat agagccaaag atatttgccg caacttcttg ggccattggt
      901 acgactcata cgtgaatgcc accaagattt ttgacgactc tgaccaaaca aaaggttacg
      961 acttcaataa aggtcaatgg cgtgattttg tgttgaaagg ccacaaagac accaatcgcc
     1021 gaattgatta cagctacgaa atcaacccag tagggacgcc tgaagagtgt atcgcgatta
     1081 tccagcaaga tattgatgcg acgggtattg acaatatttg ttgtggtttt gaagcaaacg
     1141 gttctgaaga agaaattatc gcatctatga agctattcca gtctgatgtg atgccatatc
     1201 tcaaagaaaa acagtaatta atattttcta aaaggaaaga gacatgaaat ttggattatt
     1261 cttcctcaat tttatgaatt caaagcgttc ttctgatcaa gtcatcgaag aaatgttaga
     1321 taccgcacat tacgtagatc agttgaagtt tgacacgttg gctgtttacg aaaaccattt
     1381 ctcgaacaat ggtgtggttg gtgccccact aacagtggct ggttttttac ttggtatgac
     1441 aaagaacgcc aaagtggctt cgttgaatca cgtcattacc acgcatcatc cagtacgtgt
     1501 ggcggaagaa gcgtgtctac ttgaccaaat gagtgaaggc cgttttgcct ttggctttag
     1561 tgattgtgaa aagagtgcag atatgcgctt ctttaatcga ccaacggatt ctcagtttca
     1621 gttgttcagt gagtgtcaca agatcatcaa tgatgcattc actactgggt actgccatcc
     1681 aaacaatgat ttttatagtt ttcctaaaat ctccgttaac ccacacgcgt tcactgaagg
     1741 cggtcctgcg caatttgtga atgcgacgag caaagaagtg gttgaatggg cggctaagtt
     1801 agggcttcca ctcgtgttta gatgggacga ctcaaacgct caaagaaaag aatacgccgg
     1861 tttgtaccac gaagttgctc aggcacatgg tgtcgatgtt agtcaggttc gacacaagct
     1921 gacgctgctg gtcaaccaaa atgtagatgg tgaagcagca agggcagaag ctcgcgtgta
     1981 tttggaagag tttgtccgtg aatcttactc aaataccgac tttgagcaaa aaatgggaga
     2041 gctgttgtca gaaaatgcca tcggtactta tgaagaaagt actcaggcag cgcgagttgc
     2101 gattgagtgt tgtggtgccg cggacctatt gatgtctttt gagtcgatgg aagataaagc
     2161 gcagcaaaga gcggttatcg atgtggtaaa cgccaacatc gtcaaatacc actcgtaacg
     2221 tttaactgat gctgaagggg cagcgatgcc ccttatatca ccattctttt cgccgatagc
     2281 gctaactaat agaggcattt atatggacgt actttcagcg gttaagcagg aaaacatcgc
     2341 agcgagcaca gaaatcgatg acttgatttt catgggaact cctcagcaat ggtcattgca
     2401 ggaacaaaaa caggggtacc gagctcgaat ttcgagcttc tggagcagga agatgtcgcg
     2461 ggcattagca ccagcggtct gccaagcctc cgccagccgt tgggtccctt ccgcttgagc
     2521 ttttccatct tcgacgatac gggcggcggc cccccgcgct tccgcgatcg cccgtttaca
     2581 agctgcctca gctggggcga tcacatcggc ttgaagttgc tgctgcacct gtttgatccg
     2641 ctcctgctgc acagggagtt ctgcttggct acgagcgact tcggtagcaa tgtccgcttc
     2701 agcttcggcc accaccgctt cgcgccgcgt caacgcatcc tgaatccggc gctcggcctc
     2761 ggcttgggcg atcgctacat cgcgatcgat ccgacgcagg gccgtgatct tgtcattttc
     2821 ggccgtttgg atcgcagagg cagcctgggc atcggcttca gcaattcggg catctcgctg
     2881 cagatcagcc cgctgcttgc gtccactagc cgagagataa ccgacctcat cggaaatgtt
     2941 ctggacttgc agcgtatcga ggactagacc cagctgctca aggtcatcct ccgcctcttc
     3001 cagcagactt ttggcaaagg caattttgtc ctcgttgatc tgctccggcg tgaggctggc
     3061 taaaacacca cgcaagttgc cttcgagggt ctccttggca atttgctcga tttccttacg
     3121 gtttttgcca agcagccgct cgatcgcgtt gtggatggtc ggttcttccc cagcaatctt
     3181 gatattggca acgccttcaa cagtcagggg aatgccgccc ttggagaagg cattggaaac
     3241 gcgcaactca atgatcatgt tggtcagatc catgcggagc gctttttcca gcagaggtac
     3301 ccgcaggctg ctgccgccct tgaccaagcg atagccaact cggcggccat cactactgcg
     3361 gcgactactg ccagcaaaga tcaaaatttc actgggttgg cagatgtagt agagattgcg
     3421 caggactaag ctgccagccc cggcgactca ccagtcacag aaaagcatct tacggatggc
     3481 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3541 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3601 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3661 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     3721 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3781 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3841 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3901 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3961 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4021 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4081 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4141 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4201 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4261 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4321 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4381 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4441 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4501 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4561 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4621 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4681 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4741 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4801 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4861 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4921 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     4981 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     5041 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     5101 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     5161 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     5221 ggcaggatcg atccggcagc cggcggagcg ctgctttctt ggcaagcggt cgccagcccc
     5281 aacgccaggg ctgccagccc gaaacagcgg ggcaaggcag cttggaaggg cgatcgcagc
     5341 acgggcatgg caatgtctct ctgaaggaat gcagacctta ttcgtacagc cagggttgaa
     5401 tcgtgggggt ccaatcactt agctctgctg ggctaaacca gagagcaatt tcctgttgtg
     5461 ctgtttcgat tgcatccgag ccatggatga tgttgcggcc aatattgaca ccaaaatcac
     5521 cacggatggt gcccggttct gccgtcagcg gattggtagc gccgatcaac ttgcgagcag
     5581 ccgccacaac gccttcgcct tccaagacga tcgccacgat cggcccagag gtgatgaact
     5641 cgacgaggcc attgaagaag gggcgctcgc ggtggacagc atagtgctgt tcggccagct
     5701 cgcgactggg cttcagctgc tttaggccca ccagtttgaa gcctttttgc tcaaagcggc
     5761 cgatgatcgt accgaccaaa ccccgctgaa cgccatcggg cttgatggca ataaatgtgc
     5821 gttccacaga catctagata gtcctcaaga cgaggcaagc attgagcttg ccttcctatg
     5881 gttcgggatc actgggattc ttgacaagcg atcgcggtca catcgctatc tcttaggact
     5941 tcgcagcggg cgagtcggat tgacccggta gggatttcgc cagatcaatg cccgtggttt
     6001 gtttcagctt ctccagcaag ctagcgattt gggtagcgct gccttcccct tcgccaatca
     6061 cagtgatcga ctccacgtcg atatctggca cggtgcctga aagcgtgacg agcagggact
     6121 cgaagcttgc atgcctgcag gtcgactcta gagctttatg cttgtaaacc gttttgtgaa
     6181 aaaattttta aaataaaaaa ggggacctct agggtcccca attaattagt aatataatct
     6241 attaaaggtc attcaaaagg tcatccaccg gatcaattcc cctgctcgcg caggctgggt
     6301 gccaagctct cgggtaacat caaggcccga tccttggagc ccttgccctc ccgcacgatg
     6361 atcgtgccgt gatcgaaatc cagatccttg acccgcagtt gcaaaccctc actgatccgc
     6421 atgcccgttc catacagaag ctgggcgaac aaacgatgct cgccttccag aaaaccgagg
     6481 atgcgaacca cttcatccgg ggtcagcacc accggcaagc gccgcgacgg ccgaggtctt
     6541 ccgatctcct gaagccaggg cagatccgtg cacagcacct tgccgtagaa gaacagcaag
     6601 gccgccaatg cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc cagccaggac
     6661 agaaatgcct cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg
     6721 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     6781 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     6841 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     6901 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     6961 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc
     7021 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     7081 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     7141 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     7201 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     7261 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     7321 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     7381 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     7441 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     7501 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     7561 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     7621 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     7681 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     7741 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     7801 acaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact
     7861 aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc
     7921 gtgcataata agccctacac aaattgggag atatatcatg aaaggctggc tttttcttgt
     7981 tatcgcaata gttggcgaag taatcgcaac atccgcatta aaatctagcg agggctttac
     8041 taagctgatc cggtggatga ccttttgaat gacctttaat agattatatt actaattaat
     8101 tggggaccct agaggtcccc ttttttattt taaaaatttt ttcacaaaac ggtttacaag
     8161 catactagag gatcggc
//
