LOCUS       sequence-51691-e         796 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS3
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             438..455
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(508..526)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      522..569
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(693..712)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaaatatttt gtttacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgcgcta ccgccagatc
      121 accatcccga ggggaaagga cggctttggc ttcaccatct gctgcgactc tccagttcga
      181 gtccaggccg tggattccgg gggtccggcg gaacgggcag ggctgcagca gctggacacg
      241 gtgctgcagc tgaatgagag gcctgtggag cactggaaat gtgtggagct ggcccacgag
      301 atccggagct gccccagtga gatcatccta ctcgtgtggc gcatggtccc ccaggtcaag
      361 ccaggaccag attgacagta aaggtggata cggatccgaa ttcgagctcc gtcgacaagc
      421 ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
      481 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      541 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
      601 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      661 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      721 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      781 atttagtgct ttacgg
//
