LOCUS       sequence-51736-e         865 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DLG2
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             455..472
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(525..543)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      539..586
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(710..729)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctctgaatat tttgtttact ttagaaggag atatacatat gcaccatcat catcatcatt
       61 cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgacc gttgtggaaa
      121 tcaaactgtt caaaggccct aaaggtttag gcttcagtat tgcaggaggt gtggggaacc
      181 aacacattcc tggagacaac agcatttatg taactaaaat tatagatgga ggagctgcac
      241 aaaaagatgg aaggttgcaa gtaggagata gactactaat ggtaaacaac tacagtttag
      301 aagaagtaac acacgaagag gcagtagcaa tattaaagaa cacatcagag gtagtttatt
      361 taaaagttgg caaacccact accatttatt gacagtaaag gtggatacgg atccgaattc
      421 gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
      481 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      541 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      601 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      661 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      721 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aatcgggggc
      781 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
      841 gtgatggttc acgtagtggc tcgct
//
