LOCUS       sequence-51755-e         716 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Austin BP, Waugh DS
  TITLE     Isolation of Metarhizium anisopliae carboxypeptidase A with native 
            disulfide bonds from the cytosol of Escherichia coli BL21(DE3).
  JOURNAL   Protein Expr Purif. 2012 Mar;82(1):116-24. Epub 2011 Dec 14.
  PUBMED    22197595
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2012-03"; volume: "82(1)"; pages: "116-24. Epub
            2011 Dec 14"
COMMENT     Sequence Label: pBA2219
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..43
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagatatacc atggctagca tgactggtgg acagcaaatg ggtcggatca tcacaagttt
       61 gtacaaaaaa gttgtggacg acgcggcaat tcaacaaacg ttagccaaaa tgggcatcaa
      121 aagcagcgat attcagcccg cgcctgtagc tggcatgaag acagttctga ctaacagcgg
      181 cgtgttgtac atcaccgatg atggtaaaca tatcattcag gggccaatgt atgacgttag
      241 tggcacggct ccggtcaatg tcaccaataa gatgctgtta aagcagttga atgcgcttga
      301 aaaagagatg atcgtttata aagcgccgca ggaaaaacac gtcatcaccg tatttactga
      361 tattacctgt ggttactgcc acaaactgca tgagcaaatg gcagactaca acgcgctggg
      421 gatcaccgtg cgttatcttg ctttcccgcg ccaggggctg gacagcgatg cagagaaaga
      481 aatgaaagct atctggtgtg cgaaagataa aaacaaagcg tttgatgatg tgatggcagg
      541 taaaagcgtc gcaccagcca gttgcgacgt ggatattgcc gaccattacg cacttggcgt
      601 ccagcttggc gttagcggta ctccggcagt tgtgctgagc aatggcaccc ttgttccggg
      661 ttaccagccg ccgaaagaga tgaaagaatt cctcgacgaa caccaaaaaa tgacca
//
