LOCUS       sequence-51851-e         875 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CRYBB3
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(638..658)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(683..700)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             711..733
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    748..772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(772..791)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(773..791)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg
       61 gagctcgaat tcggatccgt atccaccttt actgtcagtc acggatgcgg cgcacagact
      121 gcagctgcgg ctggctggcg tcccactcat tccagtggcg gtactcgccc cgctcaaaca
      181 cgtactggcg cccacggtag ccggggaact catagccaac ccacgtcccg ttgatggcac
      241 ggacactcgc cacacggtcc tggaagccat gagcccacag gctgggcacg tcatcatcca
      301 ctatctccat cttgcggcca ctgaaagctg ggttctcaaa cagatgcagc ttgtgatgtg
      361 gactatcaat attcagaggc cggagggaca gaaggctgtc actatcacgg ctgttggacc
      421 aggcatccca gcgaggataa tcccccttct ccagaacaaa ctgctccccg cggaaggccc
      481 tggactcaaa tgccagccac ggcccggact ccacttggat ggagcccacc ttctccagca
      541 ggctgtcggt caggctgggg cactcggccg agagctcgca gcgtttgcct tggaagttct
      601 ctagttcgta caagatcacc ttgtagctgc cccccatgga ttggaagtac aggttctcgg
      661 tacccagatc tacaccagaa gaatgatgat gatgatggtg catatgtata tctccttctt
      721 aaagttaaac aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt
      781 gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct acgccggacg catcgtggcc
      841 ggcatcaccg gcgccacagg tgcggttgct ggcgc
//
