LOCUS       sequence-51853-e         879 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GLRX3
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..41)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(422..442)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(467..484)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             495..517
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    532..556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(556..575)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(557..575)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(659..678)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggcttggtag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc
       61 ttgtcgacgg agctcgaatt cggatccgta tccaccttta ctgtcaattt tctcctctca
      121 gtataggcag caattcacca ttttctttca gttccttcac aatatccaat cctcccacca
      181 gctccccttt cacatacagc tgagggtatg ttggccaatt tgagtaagct tttaatcctt
      241 gccgaacttc ttcatcctcc aatatatcga atgtttcata ttcaacacca gtactattta
      301 gtatttccag aatttgtttg ctgaatccac attttgcttc ctgtttgttt cctttcataa
      361 agagcatcac agaagcttta tttgtcagca ctttgagcct ttcctctaat ttgggagcca
      421 tggattggaa gtacaggttc tcggtaccca gatctacacc agaagaatga tgatgatgat
      481 ggtgcatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt
      541 atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc
      601 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
      661 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
      721 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
      781 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
      841 ttcctaatgc aggagtcgca taagggagag cgtcgagat
//
