LOCUS       sequence-51892-e         906 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PBRM1
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             463..483
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             484..501
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             502..525
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             575..592
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(645..663)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      659..706
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(830..849)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta ccttaagaag gagatatact atgggtgata gcatgatcag cagcgcgacc
       61 agcgataccg gcagcgcgaa acgtaaaagc aaaaaaaata tccgtaaaca gcgcatgaaa
      121 attctgttta acgtggtgct ggaagcccgt gaaccgggca gcggtcgccg cctgtgtgat
      181 ctgtttatgg tgaaaccgag caaaaaagat tatccggatt attataaaat catcctggaa
      241 ccgatggatc tgaaaattat tgaacacaat atccgtaacg ataaatatgc cggcgaagaa
      301 ggtatgattg aagatatgaa actgatgttt cgtaacgccc gtcactataa cgaagaaggt
      361 agccaggtgt ataatgatgc gcatatcctg gaaaaactgc tgaaagaaaa acgcaaagaa
      421 ctgggtccgc tgccggatga tgatgatatg gcgagcccgg cagagaacct ctacttccaa
      481 tcgcaccatc atcaccacca tgattacaag gatgacgacg ataagtgagg atccgaattc
      541 gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
      601 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      661 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      721 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      781 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      841 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc cgtcaagctc taaatcgggg
      901 ggctcc
//
