LOCUS       sequence-51922-e         644 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 644)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SMARCA4
FEATURES             Location/Qualifiers
     source          1..644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             532..549
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(602..620)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attttgttta ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
       61 tgtagatctg ggtaccgaga acctgtactt ccaatccatg gcggaaaaac tgagcccgaa
      121 tccgccgaac ctgaccaaaa aaatgaaaaa aattgttgat gcggtgatta aatataaaga
      181 tagcagcagc ggtcgtcagc tgagcgaagt gtttattcag ctgccgagcc gtaaagaact
      241 gccggaatat tatgaactga ttcgtaaacc ggtggatttt aaaaaaatta aagaacgtat
      301 tcgcaatcat aaatatcgta gcctgaatga tctggaaaaa gatgtgatgc tgctgtgcca
      361 gaatgcgcag acctttaacc tggaaggcag cctgatttat gaagatagca ttgttctgca
      421 gagcgtgttt accagcgtgc gccagaaaat cgaaaaagaa gatgattgac agtaaaggtg
      481 gatacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac
      541 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      601 accgctgagc aataactagc ataacccctt ggggcctcta aacg
//
