LOCUS       sequence-52015-e         831 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses EGFP-Cre fusion under the control of the EF-1 alpha 
            promoter; EGFP is human codon-optimized..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Le Y, Miller JL, Sauer B
  TITLE     GFPcre fusion vectors with enhanced expression.
  JOURNAL   Anal Biochem. 1999 Jun 1. 270(2):334-6.
  PUBMED    10334853
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 1999 Jun 1. 270(2):334-6."
COMMENT     Sequence Label: pBS598 EF1alpha-EGFPcre
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(24..26,27..29,30..740)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(24..26,27..29,30..740)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(69..90)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(330..349)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     677..698
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
       61 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
      121 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      181 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
      241 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      301 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
      361 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
      421 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
      481 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
      541 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
      601 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
      661 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
      721 gcatggacga gctgtacaag tccggtacag ctctcgacgg agaaagctca ggctctggct
      781 cagagtctga ctccatggcc aatttactga ccgtacacca aaatttgcct g
//
