LOCUS       sequence-52049-e       10155 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   MJFF
  TITLE     Michael J Fox Foundation plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBACgus4x/cdc37/LRRK2 1867-2161_C2024S/C2025S
FEATURES             Location/Qualifiers
     source          1..10155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(665..774)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        complement(925..1016)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        1041..1150
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        1256..1347
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             1360..1383
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1384..1404
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2315..2332
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     2369..3726
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(2385..3726)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     primer_bind     complement(3818..3835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3989..4577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4069..4088)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4748..5539,5540..5608))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5371..5390
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5609..5713)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5740..6195
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5827..5846)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6037..6058
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_recomb     6594..7421
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             6594..6718
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(6756..7361)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(7432..9243)
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFSLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     promoter        complement(9252..9567)
                     /gene="Autographa californica p6.9"
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
ORIGIN
        1 ataaattgca tgacgattgt ctggtgggcc acctgctcca tgagtgcaca tttctcctcc
       61 acctctaggt caatgcacca aatgaccagg taattggctg tctcctcgca caccaggtgg
      121 acgttgtctg acaggtactt ttggctgtca tcccagcggc gaagcatgcc aaagtgcttg
      181 atctgtttct cgtatttttc cacgaaggtc ttgtgtttct gctccctcac ctcctctgag
      241 tcctcctccg tcttctcggg cttggtattt accatgctct tgctgaagcc gtctttgctg
      301 agcgtgtcca cgttccaggg catgctcttc tccttcttgc gcatctcctc cagcttctgc
      361 tcccagctcc gctcctcctt gcgcagctgc tgtgcctcgg cctgcaggcg ctccagctct
      421 gccttgccgc cctcggccac ctccagctcc ttcagtttcc tctggcactc ggccaccttg
      481 cgcttgcact cgcggcagcc cctgtccagt tcctccttct ccttctggaa ctgctccatg
      541 cgttccaccc gggcctgatg ccgccagcgg aagagactgg ccgtgtcgat gttggggtgc
      601 gtctcgtctt catcatcaga cacctcaatg tggtcccaca cgctgtagtc caccatggat
      661 ctgtgattgt aaataaaatg taatttacag tatagtattt taattaatat acaaatgatt
      721 tgataataat tcttatttaa ctataatata ttgtgttggg ttgaattaaa ggtcccggca
      781 tcctcaaatg cataatttca tagtccccct tgttgtaagt gatgcgtatt tctgaatctt
      841 tgtaaaatag cacacaagac tccaacgcgt ttggcgtttt attttcttgc tcgactctag
      901 tttattaggc ctctagagat ccgtatttat aggttttttt attacaaaac tgttacgaaa
      961 acagtaaaat acttatttat ttgcgagatg gttatcattt taattatctc catgatccaa
     1021 taacctagaa taaagggccc gacctttaat tcaacccaac acaatatatt atagttaaat
     1081 aagaattatt atcaaatcat ttgtatatta attaaaatac tatactgtaa attacatttt
     1141 atttacaatc acagatcccc gggactagta ataaaagatg tttattttca ttagatgtgt
     1201 gtgttggttt tttgtgtcta gtaataaaac ctagaggatc gatcccccgg atctgatcat
     1261 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
     1321 agttttgtaa taaaaaaacc tataaatatc ggatccatgc accaccatca ccaccatcac
     1381 cacgagaacc tttatttcca aggaaacatc atgctcaaca acgatgagct cgaatttgaa
     1441 caagctccag aattcctgct cggcgacggc tctttcggct ccgtataccg tgctgcttat
     1501 gaaggcgaag aagtcgccgt aaagatcttc aacaagcaca cctcgctccg tctgctccgt
     1561 caggaactgg tcgttctgtg tcatctgcac catccgagcc tcatctccct gctggccgct
     1621 ggcatccgtc ctcgtatgct ggtgatggag ctggcatcca agggctctct cgaccgcctg
     1681 ctgcagcagg acaaggcttc tctgacccgt actctgcagc atcgtatcgc tctgcatgta
     1741 gctgacggcc tgcgttatct tcattctgca atgatcattt atcgcgacct gaaacctcat
     1801 aatgtactgc tgtttactct gtacccgaac gctgccatca tcgctaagat tgcagactat
     1861 ggtattgcgc agtactcctc ccgcatgggc attaaaacaa gcgaaggtac gccgggtttc
     1921 cgtgctccag aagtggctcg tggcaacgtt atttacaacc agcaagctga cgtttactcg
     1981 ttcggtctgc tcctgtacga tatcctgact accggcggcc gtatcgtaga aggtctgaag
     2041 ttccctaacg aattcgacga actggaaatc cagggcaagc tgccagaccc ggttaaagaa
     2101 tacggttgcg ctccgtggcc gatggtagag aagctcatca aacagtgcct taaagaaaac
     2161 cctcaggaac gtccaacctc tgctcaagtg ttcgacatcc ttaacagcgc tgaactcgtc
     2221 tgtctgactc gccgtattct cctgcctaag aatgttatcg tggaatgtat ggttgctact
     2281 caccataact gataagcttg cggccgcact cgagcaccac caccaccacc actaacctag
     2341 gtagctgagc gcatgcaagc tgatccgggt tattagtaca tttattaagc gctagattct
     2401 gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt aaatttataa
     2461 tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt atatctgaat
     2521 ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc aaacaagggt
     2581 tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc cacaaaactt
     2641 gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt gttttgtaat
     2701 aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc actgtcgtta
     2761 gtgtacaatt gactcgacgt aaacacgtta aatagagctt ggacatattt aacatcgggc
     2821 gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct
     2881 tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc taacacgtcc
     2941 gcgatcaaat ttgtagttga gctttttgga attatttctg attgcgggcg tttttgggcg
     3001 ggtttcaatc taactgtgcc cgattttaat tcagacaaca cgttagaaag cgatggtgca
     3061 ggcggtggta acatttcaga cggcaaatct actaatggcg gcggtggtgg agctgatgat
     3121 aaatctacca tcggtggagg cgcaggcggg gctggcggcg gaggcggagg cggaggtggt
     3181 ggcggtgatg cagacggcgg tttaggctca aatgtctctt taggcaacac agtcggcacc
     3241 tcaactattg tactggtttc gggcgccgtt tttggtttga ccggtctgag acgagtgcga
     3301 tttttttcgt ttctaatagc ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt
     3361 tgaggttccg tcggcattgg tggagcgggc ggcaattcag acatcgatgg tggtggtggt
     3421 ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg gcggcggtgc cgccggtata
     3481 atttgttctg gtttagtttg ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc
     3541 tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta
     3601 taattggaat acaaatcgta aaaatctgct ataagcattg taatttcgct atcgtttacc
     3661 gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag
     3721 ctcggatcga tcccgcacgc cgataacaag ccttttcatt tttactacag cattgtagtg
     3781 gcgagacact tcgctgtcgt cgaggtttaa acgcttcctc gctcactgac tcgctgcgct
     3841 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3901 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3961 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4021 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4081 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4141 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4201 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4261 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4321 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4381 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     4441 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4501 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4561 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4621 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4681 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4741 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4801 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4861 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4921 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4981 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5041 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5101 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5161 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5221 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5281 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5341 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5401 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5461 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5521 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5581 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5641 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5701 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     5761 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5821 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5881 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5941 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6001 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6061 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6121 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6181 taacgtttac aatttcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     6241 tgcgggcctc ttcgctatta cgccaggaac ggctccgccc actattaatg aaattaaaaa
     6301 ttccaatttt aaaaaacgca gcaagagaaa catttgtatg aaagaatgcg tagaaggaaa
     6361 gaaaaatgtc gtcgacatgc tgaacaacaa gattaatatg cctccgtgta taaaaaaaat
     6421 attgaacgat ttgaaagaaa acaatgtacc gcgcggcggt atgtacagga agaggtttat
     6481 actaaactgt tacattgcaa acgtggtttc gtgtgccaag tgtgaaaacc gatgtttaat
     6541 caaggctctg acgcatttct acaaccacga ctccaagtgt gtgggtgaag tcatgcatct
     6601 tttaatcaaa tcccaagatg tgtataaacc accaaactgc caaaaaatga aaactgtcga
     6661 caagctctgt ccgtttgctg gcaactgcaa gggtctcaat cctatttgta attattgaat
     6721 aataaaacaa ttataaatgc taaatttgtt ttttattaac gatacaaacc aaacgcaaca
     6781 agaacatttg tagtattatc tataattgaa aacgcgtagt tataatcgct gaggtaatat
     6841 ttaaaatcat tttcaaatga ttcacagtta atttgcgaca atataatttt attttcacat
     6901 aaactagacg ccttgtcgtc ttcttcttcg tattccttct ctttttcatt tttctcttca
     6961 taaaaattaa catagttatt atcgtatcca tatatgtatc tatcgtatag agtaaatttt
     7021 ttgttgtcat aaatatatat gtctttttta atggggtgta tagtaccgct gcgcatagtt
     7081 tttctgtaat ttacaacagt gctattttct ggtagttctt cggagtgtgt tgctttaatt
     7141 attaaattta tataatcaat gaatttggga tcgtcggttt tgtacaatat gttgccggca
     7201 tagtacgcag cttcttctag ttcaattaca ccatttttta gcagcaccgg attaacataa
     7261 ctttccaaaa tgttgtacga accgttaaac aaaaacagtt cacctccctt ttctatacta
     7321 ttgtctgcga gcagttgttt gttgttaaaa ataacagcca ttgtaatgag acgcacaaac
     7381 taatatcaca aactggaaat gtctatcaat atatagttgc tgatcagatc ctcattgttt
     7441 gcctccctgc tgcggttttt caccgaagtt catgccagtc cagcgttttt gcagcagaaa
     7501 agccgccgac ttcggtttgc ggtcgcgagt gaagatccct ttcttgttac cgccaacgcg
     7561 caatatgcct tgcgaggtcg caaaatcggc gaaattccat acctgttcac cgacgacggc
     7621 gctgacgcga tcaaagacgc ggtgatacat atccagccat gcacactgat actcttcact
     7681 ccacatgtcg gtgtacattg agtgcagccc ggctaacgta tccacgccgt attcggtgat
     7741 gataatcggc tgatgcagtt tctcctgcca ggccagaagt tctttttcca gtaccttctc
     7801 tgccgtttcc aaatcgccgc tttggacata ccatccgtaa taacggttca ggcacagcac
     7861 atcaaagaga tcgctgatgg tatcggtgtg agcgtcgcag aacattacat tgacgcaggt
     7921 gatcggacgc gtcgggtcga gtttacgcgt tgcttccgcc agtggcgcga aatattcccg
     7981 tgcaccttgc ggacgggtat ccggttcgtt ggcaatactc cacatcacca cgcttgggtg
     8041 gtttttgtca cgcgctatca gctctttaat cgcctgtaag tgcgcttgct gagtttcccc
     8101 gttgactgcc tcttcgctgt acagttcttt cggcttgttg cccgcttcga aaccaatgcc
     8161 taaagagagc gaaaagccga cagcagcagt ttcatcaatc accacgatgc catgttcatc
     8221 tgcccagtcg agcatctctt cagcgtaagg gtaatgcgag gtacggtagg agttggcccc
     8281 aatccagtcc attaatgcgt ggtcgtgcac catcagcacg ttatcgaatc ctttgccacg
     8341 taagtccgca tcttcatgac gaccaaagcc agtaaagtag aacggtttgt ggttaatcag
     8401 gaactgttgg cccttcactg ccactgaccg gatgccgacg cgaagcgggt agatatcaca
     8461 ctctgtctgg cttttggctg tgacgcacag ttcatagaga taaccttcac ccggttgcca
     8521 gaggtgcgga ttcaccactt gcaaagtccc gctagtgcct tgtccagttg caaccacctg
     8581 ttgatccgca tcacgcagtt caacgctgac atcaccattg gccaccacct gccagtcaac
     8641 agacgcgtgg ttacagtctt gcgcgacatg cgtcaccacg gtgatatcgt ccacccaggt
     8701 gttcggcgtg gtgtagagca ttacgctgcg atggattccg gcatagttaa agaaatcatg
     8761 gaagtaagac tgctttttct tgccgttttc gtcggtaatc accattcccg gcgggatagt
     8821 ctgccagttc agttcgttgt tcacacaaac ggtgatacgt acacttttcc cggcaataac
     8881 atacggcgtg acatcggctt caaatggcgt atagccgccc tgatgctcca tcacttcctg
     8941 attattgacc cacactttgc cgtaatgagt gaccgcatcg aaacgcagca cgatacgctg
     9001 gcctgcccaa cctttcggta taaagacttc gcgctgatac cagacgttgc ccgcataatt
     9061 acgaatatct gcatcggcga actgatcgtt aaaactgcct ggcacagcaa ttgcccggct
     9121 ttcttgtaac gcgctttccc accaacgctg atcaattcca cagttttcgc gatccagact
     9181 gaatgcccac aggccgtcga gttttttgat ttcacgggtt ggggtttcta caggacggac
     9241 catatttata ggtgtaattt atgtagctgt aatttttacc ttattaatat tttttacgct
     9301 ttgcattcga cgactgaact cccaaatata tgtttaactc gtcttggtcg tttgaatttt
     9361 tgttgctgtg tttcctaata ttttccatca ccttaaatat gttattgtaa tcctcaatgt
     9421 tgaacttgca attggacacg gcatagtttt ccatagtcgt gtaaaacatg gtattggctg
     9481 cattgtaata catccgactg agcgggtacg gatctatgtg tttgagcagc ctgttcaaaa
     9541 actctgcatc gtcgcaaaac ggaatttcgt cgacagatcg taaccacaca aaaaaccaac
     9601 acacagttct aatgaaaata aagaactttt attatcctca ggtctagctg gaattcttat
     9661 caggtttcag gctcgggcac actgacatcc ttctcatcgc ccgtcttggg aacagcttcc
     9721 agtaatgggt ccccaggacc tgcctcctct ccctccttgg cctcgctggc cttagagttg
     9781 gggacccaga ggccagagtc aatgcagcgc tgcatgtggt actttgcgtc ggtggggtcc
     9841 atcttgctga tggcgtcctg cagcatctgc acgtccttca catcgaagca cttctggagt
     9901 tcctcaggga gggactcgta gacctcgacg gggtccaggc cgccggggcc gagccgcttc
     9961 ttgcgctcct cctcctcgta ctccttcatg gccttctcga tgcgcagctt ggcacggccc
    10021 cgcacacgct ccttgaaggc ttccagctcg tcgttgaagc cctccatgta ctggcgatcg
    10081 gctgtcttaa tcttagtgaa gaactgccgg aagcaggccc gggggtccac ctttaggctc
    10141 ttggccagct ccagg
//
