LOCUS       sequence-52348-e         867 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Sinha H, Nicholson BP, Steinmetz LM, McCusker JH
  TITLE     Complex genetic interactions in a quantitative trait locus.
  JOURNAL   PLoS Genet. 2006 Feb;2(2):e13. Epub 2006 Feb 3.
  PUBMED    16462944
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2006-02"; volume: "2(2)"; pages: "e13. Epub 2006 Feb
            3"
COMMENT     Sequence Label: pBN98
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     496..512
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     496..512
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(546..562)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(547..563)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(591..609)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(630..646)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(630..646)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(643..665)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    654..670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(678..684,685..702,703..708))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    723..744
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aatcgcggcc tcgaaacgtg agtcttttcc ttacccatgg ttgtgtacaa tgttcggatg
       61 tgatgtgaga actgtatcct agcaagattt taaaaggaag tatatgaaag aagaacctca
      121 gtggcaaatc ctaacctttt atatttctct acaggggcgc ggcgtgggga caattcaacg
      181 cgtctgtgag gggagcgttt ccctgctcgc aggtccgcag cgaggagccg taatttttgc
      241 ttcgcgccgt gcggccatca aaatgtatgg atgcaaatga ttatacatgg ggatgtatgg
      301 gctaaatgta cgggcgacag tcacatcatg cccctgagct gcgcacgtca agactgtcaa
      361 ggagggtatt ctgggcctcc atgtcgctgg ccgggtgacc cggcggggac gaggcaagct
      421 aaacagatct ggcgcgcctt aattaacccg gggatccgtc gacctgcagc gtacgaagct
      481 tcagctggcg gccgctctag aactagtgga tcccccgggc tgcaggaatt cgatatcaag
      541 cttatcgata ccgtcgacct cgaggggggg cccggtacca gcttttgttc cctttagtga
      601 gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
      661 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      721 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      781 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      841 attgggcgct cttccgcttc ctcgctc
//
