LOCUS       sequence-52401-e         861 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Rodrigo G, Landrain TE, Jaramillo A
  TITLE     De novo automated design of small RNA circuits for engineering 
            synthetic riboregulation in living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15271-6. Epub 2012 Sep
            4.
  PUBMED    22949707
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15271-6. Epub 2012 Sep 4"
COMMENT     Sequence Label: pRAJ11m
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             63..74
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    121..137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    169..190
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      193..236
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(240..258)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        326..430
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(649..668)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcaacaagaa ttgggacaac tccagtgaaa agttcttctc ctttacggct agtcattcat
       61 aatttctcct ctttaacggg aggattgaag aagtgaaatt atgcgagggt gctcagtatc
      121 ttgttatccg ctcacaatgt caatgttatc cgctcacatt tatgctagct ctagaagcgg
      181 ccgcgaattc cagaaatcat ccttagcgaa agctaaggat tttttttatc tgaaattctg
      241 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      301 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      361 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      421 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      481 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      541 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      601 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      661 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      721 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      781 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      841 gacaacgatc ggaggaccga a
//
