LOCUS       sequence-52556-e       10866 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10866)
  AUTHORS   Hughes RM, Bolger S, Tapadia H, Tucker CL
  TITLE     Light-mediated control of DNA transcription in yeast.
  JOURNAL   Methods. 2012 Aug 15.
  PUBMED    22922268
REFERENCE   2  (bases 1 to 10866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2012 Aug 15."
COMMENT     Sequence Label: pRMH120
FEATURES             Location/Qualifiers
     source          1..10866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    15..31
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     20..42
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     39..55
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     39..55
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     74..94
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        76..94
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        112..510
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             526..966
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             982..2475
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     terminator      2482..2729
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2498..2516)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        3507..4212
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4258..4278
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4294..4635
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     promoter        4641..4659
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4684..5193
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     terminator      6087..6274
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6142..6166)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        complement(6301..6319)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6329..6346)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6329..6345)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6338..6360)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6490..6945
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6577..6596)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6787..6808
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7044..7718)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(7719..7999)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(8016..8035)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8135..8157
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8195..8213)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    8255..8758
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        8795..8899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8900..8968,8969..9760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9118..9137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9931..10519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10420..10439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10673..10690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(10843..10848,10849..10866,1..7)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
       61 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tcatagcttc
      121 aaaatgtttc tactcctttt ttactcttcc agattttctc ggactccgcg catcgccgta
      181 ccacttcaaa acacccaagc acagcatact aaatttcccc tctttcttcc tctagggtgt
      241 cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga gaccgcctcg tttctttttc
      301 ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt tttcttgaaa attttttttt
      361 tgattttttt ctctttcgat gacctcccat tgatatttaa gttaataaac ggtcttcaat
      421 ttctcaagtt tcagtttcat ttttcttgtt ctattacaac tttttttact tcttgctcat
      481 tagaaagaaa gcatagcaat ctaatctaag ttttctagaa ctagtatgaa gctactgtct
      541 tctatcgaac aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa
      601 ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa
      661 aggtctccgc tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa
      721 cagctatttc tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct
      781 ttacaggata taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat
      841 gccgtcacag atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat
      901 agaataagtg cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact
      961 gtatcgccgc tcgaggccac catgaagatg gacaaaaaga ctatagtttg gtttagaaga
     1021 gacctaagga ttgaggataa tcctgcatta gcagcagctg ctcacgaagg atctgttttt
     1081 cctgtcttca tttggtgtcc tgaagaagaa ggacagtttt atcctggaag agcttcaaga
     1141 tggtggatga aacaatcact tgctcactta tctcaatcct tgaaggctct tggatctgac
     1201 ctcactttaa tcaaaaccca caacacgatt tcagcgatct tggattgtat ccgcgttacc
     1261 ggtgctacaa aagtcgtctt taaccacctc tatgatcctg tttcgttagt tcgggaccat
     1321 accgtaaagg agaagctggt ggaacgtggg atctctgtgc aaagctacaa tggagatcta
     1381 ttgtatgaac cgtgggagat atactgcgaa aagggcaaac cttttacgag tttcaattct
     1441 tactggaaga aatgcttaga tatgtcgatt gaatccgtta tgcttcctcc tccttggcgg
     1501 ttgatgccaa taactgcagc ggctgaagcg atttgggcgt gttcgattga agaactaggg
     1561 ctggagaatg aggccgagaa accgagcaat gcgttgttaa ctagagcttg gtctccagga
     1621 tggagcaatg ctgataagtt actaaatgag ttcatcgaga agcagttgat agattatgca
     1681 aagaacagca agaaagttgt tgggaattct acttcactac tttctccgta tctccatttc
     1741 ggggaaataa gcgtcagaca cgttttccag tgtgcccgga tgaaacaaat tatatgggca
     1801 agagataaga acagtgaagg agaagaaagt gcagatcttt ttcttagggg aatcggttta
     1861 agagagtatt ctcggtatat atgtttcaac ttcccgttta ctcacgagca atcgttgttg
     1921 agtcatcttc ggtttttccc ttgggatgct gatgttgata agttcaaggc ctggagacaa
     1981 ggcaggaccg gttatccgtt ggtggatgcc ggaatgagag agctttgggc taccggatgg
     2041 atgcataaca gaataagagt gattgtttca agctttgctg tgaagtttct tctccttcca
     2101 tggaaatggg gaatgaagta tttctgggat acacttttgg atgctgattt ggaatgtgac
     2161 atccttggct ggcagtatat ctctgggagt atccccgatg gccacgagct tgatcgcttg
     2221 gacaatcccg cgttacaagg cgccaaatat gacccagaag gtgagtacat aaggcaatgg
     2281 cttcccgagc ttgcgagatt gccaactgaa tggatccatc atccatggga cgctccttta
     2341 accgtactca aagcttctgg tgtggaactc ggaacaaact atgcgaaacc cattgtagac
     2401 atcgacacag ctcgtgagct actagctaaa gctatttcaa gaacccgtga agcacagatc
     2461 atgatcggag cagcagcccg gtcatgtaat tagttatgtc acgcttacat tcacgccctc
     2521 cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta
     2581 tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt
     2641 tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg
     2701 ttttgggacg ctcgaaggct ttaatttgcg gccggtacca tgcctgcagg tcgagatccg
     2761 ggatcgaaga aatgatggta aatgaaatag gaaatcaagg agcatgaagg caaaagacaa
     2821 atataagggt cgaacgaaaa ataaagtgaa aagtgttgat atgatgtatt tggctttgcg
     2881 gcgccgaaaa aacgagttta cgcaattgca caatcatgct gactctgtgg cggacccgcg
     2941 ctcttgccgg cccggcgata acgctgggcg tgaggctgtg cccggcggag ttttttgcgc
     3001 ctgcattttc caaggtttac cctgcgctaa ggggcgagat tggagaagca ataagaatgc
     3061 cggttggggt tgcgatgatg acgaccacga caactggtgt cattatttaa gttgccgaaa
     3121 gaacctgagt gcatttgcaa catgagtata ctagaagaat gagccaagac ttgcgagacg
     3181 cgagtttgcc ggtggtgcga acaatagagc gaccatgacc ttgaaggtga gacgcgcata
     3241 accgctagag tactttgaag aggaaacagc aatagggttg ctaccagtat aaatagacag
     3301 gtacatacaa cactggaaat ggttgtctgt ttgagtacgc tttcaattca tttgggtgtg
     3361 cactttatta tgttacaata tggaagggaa ctttacactt ctcctatgca catatattaa
     3421 ttaaagtcca atgctagtag agaagggggg taacacccct ccgcgctctt ttccgatttt
     3481 tttctaaacc gtggaatatt tcggatatcc ttttgttgtt tccgggtgta caatatggac
     3541 ttcctctttt ctggcaacca aacccataca tcgggattcc tataatacct tcgttggtct
     3601 ccctaacatg taggtggcgg aggggagata tacaatagaa cagataccag acaagacata
     3661 atgggctaaa caagactaca ccaattacac tgcctcattg atggtggtac ataacgaact
     3721 aatactgtag ccctagactt gatagccatc atcatatcga agtttcacta ccctttttcc
     3781 atttgccatc tattgaagta ataataggcg catgcaactt cttttctttt tttttctttt
     3841 ctctctcccc cgttgttgtc tcaccatatc cgcaatgaca aaaaaaatga tggaagacac
     3901 taaaggaaaa aattaacgac aaagacagca ccaacagatg tcgttgttcc agagctgatg
     3961 aggggtatct cgaagcacac gaaacttttt ccttccttca ttcacgcaca ctactctcta
     4021 atgagcaacg gtatacggcc ttccttccag ttacttgaat ttgaaataaa aaaaagtttg
     4081 ctgtcttgct atcaagtata aatagacctg caattattaa tcttttgttt cctcgtcatt
     4141 gttctcgttc cctttcttcc ttgtttcttt ttctgcacaa tatttcaagc tataccaagc
     4201 atacaatcaa ctccaagctt tgcaaagatg gataaagcgg aattaattcc cgagcctcca
     4261 aaaaagaaga gaaaggtcga attgggtacc gccgccaatt ttaatcaaag tgggaatatt
     4321 gctgatagct cattgtcctt cactttcact aacagtagca acggtccgaa cctcataaca
     4381 actcaaacaa attctcaagc gctttcacaa ccaattgcct cctctaacgt tcatgataac
     4441 ttcatgaata atgaaatcac ggctagtaaa attgatgatg gtaataattc aaaaccactg
     4501 tcacctggtt ggacggacca aactgcgtat aacgcgtttg gaatcactac agggatgttt
     4561 aataccacta caatggatga tgtatataac tatctattcg atgatgaaga taccccacca
     4621 aacccaaaaa aagagatctt taatacgact cactataggg cgagcgccga agctagcgcc
     4681 accatgaatg gagctatagg aggtgacctt ttgctcaatt ttcctgacat gtcggtccta
     4741 gagcgccaaa gggctcacct caagtacctc aatcccacct ttgattctcc tctcgccggc
     4801 ttctttgccg attcttcaat gattaccggc ggcgagatgg acagctatct ttcgactgcc
     4861 ggtttgaatc ttccgatgat gtacggtgag acgacggtgg aaggtgattc aagactctca
     4921 atttcgccgg aaacgacgct tgggactgga aatttcaaga aacggaagtt tgatacagag
     4981 actaaggatt gtaatgagaa gaagaagaag atgacgatga acagagatga cctagtagaa
     5041 gaaggagaag aagagaagtc gaaaataaca gagcaaaaca atgggagcac aaaaagcatc
     5101 aagaagatga aacacaaagc caagaaagaa gagaacaatt tctctaatga ttcatctaaa
     5161 gtgacgaagg aattggagaa aacggattat attcatgttc gtgcacgacg aggccaagcc
     5221 actgatagtc acagcatagc agaacgagtt agaagagaaa agatcagtga gagaatgaag
     5281 tttctacaag atttggttcc tggatgcgac aagatcacag gcaaagcagg gatgcttgat
     5341 gaaatcatta actatgttca gtctcttcag agacaaatcg agttcttatc gatgaaacta
     5401 gcaattgtga atccaaggcc ggattttgat atggatgaca tttttgccaa agaggttgcc
     5461 tcaactccaa tgactgtggt gccatctcct gaaatggttc tttccggtta ttctcatgag
     5521 atggttcact ctggttattc tagtgagatg gttaactccg gttaccttca tgtcaatcca
     5581 atgcagcaag tgaataccag ttctgatcca ttgtcatgct tcaacaatgg cgaagctcct
     5641 tcgatgtggg actctcatgt gcagaatctc tatggcaatt taggagtacc ggtcatcgag
     5701 ctcgagctgc agatgaatcg tagatactga aaaaccccgc aagttcactt caactgtgca
     5761 tcgtgcacca tctcaatttc tttcatttat acatcgtttt gccttctttt atgtaactat
     5821 actcctctaa gtttcaatct tggccatgta acctctgatc tatagaattt tttaaatgac
     5881 tagaattaat gcccatcttt tttttggacc taaattcttc atgaaaatat attacgaggg
     5941 cttattcaga agctttggac ttcttcgcca gaggtttggt caagtctcca atcaaggttg
     6001 tcggcttgtc taccttgcca gaaatttacg aaaagatgga aaagggtcaa atcgttggta
     6061 gatacgttgt tgacacttct aaataagcga atttcttatg atttatgatt tttattatta
     6121 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
     6181 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc
     6241 atgaggtcgc tcttattgac cacacctcta ccggccggtc gaaattatcg atcaattcgc
     6301 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     6361 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     6421 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     6481 aatggcgcga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6541 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6601 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6661 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6721 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6781 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6841 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6901 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcctga tgcggtattt
     6961 tctccttacg catctgtgcg gtatttcaca ccgcataggc aagtgcacaa acaatactta
     7021 aataaatact actcagtaat aacctatttc ttagcatttt tgacgaaatt tgctattttg
     7081 ttagagtctt ttacaccatt tgtctccaca cctccgctta catcaacacc aataacgcca
     7141 tttaatctaa gcgcatcacc aacattttct ggcgtcagtc caccagctaa cataaaatgt
     7201 aagctttcgg ggctctcttg ccttccaacc cagtcagaaa tcgagttcca atccaaaagt
     7261 tcacctgtcc cacctgcttc tgaatcaaac aagggaataa acgaatgagg tttctgtgaa
     7321 gctgcactga gtagtatgtt gcagtctttt ggaaatacga gtcttttaat aactggcaaa
     7381 ccgaggaact cttggtattc ttgccacgac tcatctccat gcagttggac gatatcaatg
     7441 ccgtaatcat tgaccagagc caaaacatcc tccttaggtt gattacgaaa cacgccaacc
     7501 aagtatttcg gagtgcctga actattttta tatgctttta caagacttga aattttcctt
     7561 gcaataaccg ggtcaattgt tctctttcta ttgggcacac atataatacc cagcaagtca
     7621 gcatcggaat ctagagcaca ttctgcggcc tctgtgctct gcaagccgca aactttcacc
     7681 aatggaccag aactacctgt gaaattaata acagacatac tccaagctgc ctttgtgtgc
     7741 ttaatcacgt atactcacgt gctcaatagt caccaatgcc ctccctcttg gccctctcct
     7801 tttctttttt cgaccgaatt aattcttaat cggcaaaaaa agaaaagctc cggatcaaga
     7861 ttgtacgtaa ggtgacaagc tatttttcaa taaagaatat cttccactac tgccatctgg
     7921 cgtcataact gcaaagtaca catatattac gatgctgtct attaaatgct tcctatatta
     7981 tatatatagt aatgtcgttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     8041 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     8101 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     8161 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     8221 gttaatgtca tgataataat ggtttcttag gacggatcgc ttgcctgtaa cttacacgcg
     8281 cctcgtatct tttaatgatg gaataatttg ggaatttact ctgtgtttat ttatttttat
     8341 gttttgtatt tggattttag aaagtaaata aagaaggtag aagagttacg gaatgaagaa
     8401 aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc gtactttaca tatatattta
     8461 ttagacaaga aaagcagatt aaatagatat acattcgatt aacgataagt aaaatgtaaa
     8521 atcacaggat tttcgtgtgt ggtcttctac acagacaaga tgaaacaatt cggcattaat
     8581 acctgagagc aggaagagca agataaaagg tagtatttgt tggcgatccc cctagagtct
     8641 tttacatctt cggaaaacaa aaactatttt ttctttaatt tcttttttta ctttctattt
     8701 ttaatttata tatttatatt aaaaaattta aattataatt atttttatag cacgtgatga
     8761 aaaggaccca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8821 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8881 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8941 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     9001 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     9061 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     9121 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     9181 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     9241 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     9301 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     9361 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     9421 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     9481 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     9541 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     9601 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     9661 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     9721 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9781 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9841 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9901 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9961 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    10021 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
    10081 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    10141 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    10201 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    10261 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    10321 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    10381 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    10441 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    10501 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    10561 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    10621 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    10681 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    10741 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    10801 cgcaattaat gtgagttacc tcactcatta ggcaccccag gctttacact ttatgcttcc
    10861 ggctcc
//
