LOCUS       sequence-52697-e         860 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FASN
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             22..44
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             55..72
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             97..117
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 agaaattccc tctagaataa ttttgtttaa ctttaagaag gagatataca tatgcaccat
       61 catcatcatc attcttctgg tgtagatctg ggtaccgaga acctgtactt ccaatccatg
      121 cgtctgctgc gggccagcgg acgcacccct gaggccgtgc agaagctgct ggagcagggc
      181 ctccggcaca gccaggacct ggctttcctg agcatgctga acgacatcgc ggctgtcccc
      241 gccaccgcca tgcccttccg tggctacgct gtgctgggtg gtgagcgcgg tggcccagag
      301 gtgcagcagg tgcccgctgg cgagcgcccg ctctggttca tctgctctgg gatgggcaca
      361 cagtggcgcg ggatggggct gagcctcatg cgcctggacc gcttccgaga ttccatccta
      421 cgctccgatg aggctgtgaa gccattcggc ctgaaggtgt cacagctgct gctgagcaca
      481 gacgagagca cctttgatga catcgtccat tcgtttgtga gcctgactgc catccagata
      541 ggcctcatag acctgctgag ctgcatgggg ctgaggccag atggcatcgt cggccactcc
      601 ctgggggagg tggcctgtgg ctacgccgac ggctgcctgt cccaggagga ggccgtcctc
      661 gctgcctact ggaggggaca gtgcatcaaa gaagcccatc tcccgccggg cgccatggca
      721 gccgtgggct tgtcctggga ggagtgtaaa cagcgctgcc ccccgggcgt ggtgcccgcc
      781 tgccacaact ccaaggacac agtcaccatc tcgggacctc aggccccggt gtttgagttc
      841 gtggagcagc tgaggaagga
//
