LOCUS       sequence-52732-e         760 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pFB-LIC-Bse
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             90..107
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             132..152
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(182..215)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        230..675
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 acagttttgt ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg
       61 gcgcggatct cggtccgaaa accatgggcc accatcatca tcatcattct tctggtgtag
      121 atctgggtac cgagaacctg tacttccaat ccataagcta gcttctcctc ctgaaagatc
      181 cataacttcg tatagcatac attatacgaa gttatgcggc cgcgacgtcc acatatacct
      241 gccgttcact attatttagt gaaatgagat attatgatat tttctgaatt gtgattaaaa
      301 aggcaacttt atgcccatgc aacagaaact ataaaaaata cagagaatga aaagaaacag
      361 atagattttt tagttcttta ggcccgtagt ctgcaaatcc ttttatgatt ttctatcaaa
      421 caaaagagga aaatagacca gttgcaatcc aaacgagagt ctaatagaat gaggtcgaaa
      481 agtaaatcgc gcgggtttgt tactgataaa gcaggcaaga cctaaaatgt gtaaagggca
      541 aagtgtatac tttggcgtca ccccttacat attttaggtc tttttttatt gtgcgtaact
      601 aacttgccat cttcaaacag gagggctgga agaagcagac cgctaacaca gtacataaaa
      661 aaggagacat gaacgatgaa catcaaaaag tttgcaaaac aagcaacagt attaaccttt
      721 actaccgcac tgctggcagg aggcgcaact caagcgtttg
//
