LOCUS       sequence-52733-e        9224 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses LexA-CRY2PHR for light-inducible protein expression when 
            coupled with VP16-CIB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Hughes RM, Bolger S, Tapadia H, Tucker CL
  TITLE     Light-mediated control of DNA transcription in yeast.
  JOURNAL   Methods. 2012 Aug 15.
  PUBMED    22922268
REFERENCE   2  (bases 1 to 9224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2012 Aug 15."
COMMENT     Sequence Label: pRMH122-LexA-CRY2PHR
FEATURES             Location/Qualifiers
     source          1..9224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    70..573
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     complement(801..820)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             812..1627
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCWRPRLNSNMDADLYGYKWARDNVGQSGATIFRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIRGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHTLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(885..904)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1764..2352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2506..2523
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2676..2681,2682..2699,2700..2706)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2714..2730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2719..2741
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2738..2754
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2775..2793
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(2824..3071)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     3037..3055
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        5029..5733
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             join(5761..6012,6013..6366)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     6304..6325
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     regulatory      6388..6397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6460..7953
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     terminator      7987..8174
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8042..8066)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(8227..8901)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(8902..9003)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(9018..9034)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9055..9074)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9174..9196
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatctg cagctctggc ccgtgtctca
      721 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
      781 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg
      841 ctggaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
      901 cgataatgtc gggcaatcag gtgcgacaat ctttcgattg tatgggaagc ccgatgcgcc
      961 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     1021 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     1081 tcctgatgat gcatggttac tcaccactgc gatccgcggg aaaacagcat tccaggtatt
     1141 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     1201 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     1261 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     1321 taatggctgg cctgttgaac aagtctggaa agaaatgcat acgcttttgc cattctcacc
     1381 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     1441 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     1501 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     1561 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     1621 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
     1681 ggacggcgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1741 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1801 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1861 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     1921 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1981 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2041 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2101 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     2161 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2221 ggaacaggag agcgcacgag ggagcttcca ggggggaacg cctggtatct ttatagtcct
     2281 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggccg
     2341 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2401 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2461 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2521 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2581 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2641 aatgtgagtt acctcactca ttaggcaccc caggctttac actttatgct tccggctcct
     2701 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     2761 tacgccaagc tcggaattaa ccctcactaa agggaacaaa agctggtacc gatcccgagc
     2821 tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt tcagtataat
     2881 gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata taaataacgt
     2941 tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag gttgtctaac
     3001 tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc gtgacataac
     3061 taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt ttttcaagta
     3121 ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca tcttggtact
     3181 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
     3241 tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga agtagatgtt
     3301 gaattagatt aaactgaaga tatataattt attggaaaat acatagagct ttttgttgat
     3361 gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct tcagccaact
     3421 tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc ttacccaaga
     3481 tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta gaagcagatt
     3541 tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc aagttcaaga
     3601 ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc ttaccgaaat
     3661 aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg gccatacctc
     3721 taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag acgtgacctc
     3781 tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat gattgttctg
     3841 ggatttaatg caaaaatcac ttaagaagga aaatcaacgg agaaagcaaa cgccatctta
     3901 aatatacggg atacagatga aagggtttga acctatctgg aaaatagcat taaacaagcg
     3961 aaaaactgcg aggaaaattg tttgcgtctc tgcgggctat tcacgcgcca gaggaaaata
     4021 ggaaaaataa cagggcatta gaaaaataat tttgattttg gtaatgtgtg ggtcctggtg
     4081 tacagatgtt acattggtta cagtactctt gtttttgctg tgtttttcga tgaatctcca
     4141 aaatggttgt tagcacatgg aagagtcacc gatgctaagt tatctctatg taagctacgt
     4201 ggcgtgactt ttgatgaagc cgcacaagag atacaggatt ggcaactgca aatagaatct
     4261 ggggatcccc cctcgagatc cgggatcgaa gaaatgatgg taaatgaaat aggaaatcaa
     4321 ggagcatgaa ggcaaaagac aaatataagg gtcgaacgaa aaataaagtg aaaagtgttg
     4381 atatgatgta tttggctttg cggcgccgaa aaaacgagtt tacgcaattg cacaatcatg
     4441 ctgactctgt ggcggacccg cgctcttgcc ggcccggcga taacgctggg cgtgaggctg
     4501 tgcccggcgg agttttttgc gcctgcattt tccaaggttt accctgcgct aaggggcgag
     4561 attggagaag caataagaat gccggttggg gttgcgatga tgacgaccac gacaactggt
     4621 gtcattattt aagttgccga aagaacctga gtgcatttgc aacatgagta tactagaaga
     4681 atgagccaag acttgcgaga cgcgagtttg ccggtggtgc gaacaataga gcgaccatga
     4741 ccttgaaggt gagacgcgca taaccgctag agtactttga agaggaaaca gcaatagggt
     4801 tgctaccagt ataaatagac aggtacatac aacactggaa atggttgtct gtttgagtac
     4861 gctttcaatt catttgggtg tgcactttat tatgttacaa tatggaaggg aactttacac
     4921 ttctcctatg cacatatatt aattaaagtc caatgctagt agagaagggg ggtaacaccc
     4981 ctccgcgctc ttttccgatt tttttctaaa ccgtggaata tttcggatat ccttttgttg
     5041 tttccgggtg tacaatatgg acttcctctt ttctggcaac caaacccata catcgggatt
     5101 cctataatac cttcgttggt ctccctaaca tgtaggtggc ggaggggaga tatacaatag
     5161 aacagatacc agacaagaca taatgggcta aacaagacta caccaattac actgcctcat
     5221 tgatggtggt acataacgaa ctaatactgt agccctagac ttgatagcca tcatcatatc
     5281 gaagtttcac tacccttttt ccatttgcca tctattgaag taataatagg cgcatgcaac
     5341 ttcttttctt tttttttctt ttctctctcc cccgttgttg tctcaccata tccgcaatga
     5401 caaaaaaaat gatggaagac actaaaggaa aaaattaacg acaaagacag caccaacaga
     5461 tgtcgttgtt ccagagctga tgaggggtat cttcgaacac acgaaacttt ttccttcctt
     5521 cattcacgca cactactctc taatgagcaa cggtatacgg ccttccttcc agttacttga
     5581 atttgaaata aaaaaagttt gccgctttgc tatcaagtat aaatagacct gcaattatta
     5641 atcttttgtt tcctcgtcat tgttctcgtt ccctttcttc cttgtttctt tttctgcaca
     5701 atatttcaag ctataccaag catacaatca actccaagct tgaagcaagc ctcctgaaag
     5761 atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc
     5821 cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca
     5881 aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc
     5941 ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt
     6001 cgtgtggctg ccggtgaacc acttctggcg caacagcata ttgaaggtca ttatcaggtc
     6061 gatccttcct tattcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg
     6121 aaagatatcg gcattatgga tggtgacttg ctggcagtgc ataaaactca ggatgtacgt
     6181 aacggtcagg tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa
     6241 cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgtagat
     6301 cttcgtcagc agagcttcac cattgaaggg ctggcggttg gggttattcg caacggcgac
     6361 tggctggaat tcccggggat ccgtcgacca tggcggccgc tcgagtcgac ctgcagccaa
     6421 gctaattccg ggcgaatttc ttatgattta ggcggcagca tgaagatgga caaaaagact
     6481 atagtttggt ttagaagaga cctaaggatt gaggataatc ctgcattagc agcagctgct
     6541 cacgaaggat ctgtttttcc tgtcttcatt tggtgtcctg aagaagaagg acagttttat
     6601 cctggaagag cttcaagatg gtggatgaaa caatcacttg ctcacttatc tcaatccttg
     6661 aaggctcttg gatctgacct cactttaatc aaaacccaca acacgatttc agcgatcttg
     6721 gattgtatcc gcgttaccgg tgctacaaaa gtcgtcttta accacctcta tgatcctgtt
     6781 tcgttagttc gggaccatac cgtaaaggag aagctggtgg aacgtgggat ctctgtgcaa
     6841 agctacaatg gagatctatt gtatgaaccg tgggagatat actgcgaaaa gggcaaacct
     6901 tttacgagtt tcaattctta ctggaagaaa tgcttagata tgtcgattga atccgttatg
     6961 cttcctcctc cttggcggtt gatgccaata actgcagcgg ctgaagcgat ttgggcgtgt
     7021 tcgattgaag aactagggct ggagaatgag gccgagaaac cgagcaatgc gttgttaact
     7081 agagcttggt ctccaggatg gagcaatgct gataagttac taaatgagtt catcgagaag
     7141 cagttgatag attatgcaaa gaacagcaag aaagttgttg ggaattctac ttcactactt
     7201 tctccgtatc tccatttcgg ggaaataagc gtcagacacg ttttccagtg tgcccggatg
     7261 aaacaaatta tatgggcaag agataagaac agtgaaggag aagaaagtgc agatcttttt
     7321 cttaggggaa tcggtttaag agagtattct cggtatatat gtttcaactt cccgtttact
     7381 cacgagcaat cgttgttgag tcatcttcgg tttttccctt gggatgctga tgttgataag
     7441 ttcaaggcct ggagacaagg caggaccggt tatccgttgg tggatgccgg aatgagagag
     7501 ctttgggcta ccggatggat gcataacaga ataagagtga ttgtttcaag ctttgctgtg
     7561 aagtttcttc tccttccatg gaaatgggga atgaagtatt tctgggatac acttttggat
     7621 gctgatttgg aatgtgacat ccttggctgg cagtatatct ctgggagtat ccccgatggc
     7681 cacgagcttg atcgcttgga caatcccgcg ttacaaggcg ccaaatatga cccagaaggt
     7741 gagtacataa ggcaatggct tcccgagctt gcgagattgc caactgaatg gatccatcat
     7801 ccatgggacg ctcctttaac cgtactcaaa gcttctggtg tggaactcgg aacaaactat
     7861 gcgaaaccca ttgtagacat cgacacagct cgtgagctac tagctaaagc tatttcaaga
     7921 acccgtgaag cacagatcat gatcggagca gcagcccggg tcgacctgca gccaagctaa
     7981 ttccgggcga atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata
     8041 agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt gttcttgagt
     8101 aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac
     8161 cacacctcta ccggcatgcc ggcaagtgca caaacaatac ttaaataaat actactcagt
     8221 aataacctat ttcttagcat ttttgacgaa atttgctatt ttgttagagt cttttacacc
     8281 atttgtctcc acacctccgc ttacatcaac accaataacg ccatttaatc taagcgcatc
     8341 accaacattt tctggcgtca gtccaccagc taacataaaa tgtaagcttt cggggctctc
     8401 ttgccttcca acccagtcag aaatcgagtt ccaatccaaa agttcacctg tcccacctgc
     8461 ttctgaatca aacaagggaa taaacgaatg aggtttctgt gaagctgcac tgagtagtat
     8521 gttgcagtct tttggaaata cgagtctttt aataactggc aaaccgagga actcttggta
     8581 ttcttgccac gactcatctc catgcagttg gacgatatca atgccgtaat cattgaccag
     8641 agccaaaaca tcctccttag gttgattacg aaacacgcca accaagtatt tcggagtgcc
     8701 tgaactattt ttatatgctt ttacaagact tgaaattttc cttgcaataa ccgggtcaat
     8761 tgttctcttt ctattgggca cacatataat acccagcaag tcagcatcgg aatctagagc
     8821 acattctgcg gcctctgtgc tctgcaagcc gcaaactttc accaatggac cagaactacc
     8881 tgtgaaatta ataacagaca tactccaagc tgcctttgtg tgcttaatca cgtatactca
     8941 cgtgctcaat agtcaccaat gccctccctc ttggccctct ccttttcttt tttcgaccga
     9001 attaattcgt aatcatggtc atagctgttt cctgtcgata tggtgcactc tcagtacaat
     9061 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     9121 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     9181 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcga
//
