LOCUS       sequence-52765-e         863 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: KLHL12
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             185..205
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgcaccatca tcatcatcat tcttctggtg tagatctggg
      181 taccgagaac ctgtacttcc aatccatgca gggacccagg acaagggctc gcctaggagc
      241 caatgaagtg cttttggtgg ttgggggctt tggaagccag cagtctccca ttgatgtggt
      301 agagaaatat gaccccaaga ctcaggagtg gagctttttg ccaagcatca ctcgtaagag
      361 acgttatgtg gcctcagtgt cccttcatga ccggatctac gtcattggtg gctatgatgg
      421 ccgttcccgc cttagttcag tggaatgtct agactacaca gcagatgagg atggggtctg
      481 gtattctgtg gcccctatga atgtccgacg aggtcttgct ggagccacca ccctgggaga
      541 tatgatctat gtctctggag gctttgatgg aagcaggcgt cacaccagta tggagcgcta
      601 tgatccaaac attgaccagt ggagcatgct gggagatatg cagacagccc gggaaggtgc
      661 cggactcgta gtggccagtg gagtgatcta ctgtctagga ggatatgacg gcttgaatat
      721 cttaaattca gttgagaaat acgaccctca tacaggacat tggactaatg ttacaccaat
      781 ggccaccaag cgttctggtg caggagtagc cctgctgaat gaccatattt atgtggtggg
      841 gggatttgat ggtacagccc acc
//
