LOCUS       sequence-52766-e         824 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ADH4
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             144..161
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             186..206
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggan cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacat atgcaccatc atcatcatca ttcttctggt gtagatctgg
      181 gtaccgagaa cctgtacttc caatccatgg gcaccaaggg caaagttatt aaatgcaaag
      241 cagccatcgc ctgggaagca ggcaagcccc tttgcattga agaggttgaa gtagctcccc
      301 ccaaggctca tgaagttcgc attcagatca ttgctacctc cctgtgccat actgatgcca
      361 ctgttatcga ttctaaattt gagggcctag ctttcccagt gatcgttggc catgaggctg
      421 caggtattgt ggaaagtatt gggccaggag tgaccaacgt caaaccaggt gacaaagtaa
      481 ttccacttta tgcacctcta tgtagaaaat gcaagttttg tctgagtcca ctcacaaatt
      541 tgtgtgggaa aatcagtaat ctcaaaagtc ctgctagtga tcaacaacta atggaagaca
      601 aaaccagcag gtttacctgc aaaggaaaac cagtttacca tttctttgga accagtacat
      661 tctctcagta cactgtggtg tcagatatca atcttgccaa aatagatgat gatgcaaatt
      721 tagagagagt ttgtctgctt ggatgtgggt tttcaactgg ctatggggct gcaatcaaca
      781 atgccaaggt cacccctggt tcgacttgtg ctgtctttgg ccta
//
