LOCUS       sequence-52777-e         896 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: STK17B
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             185..205
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgcaccatca tcatcatcat tcttctggtg tagatctggg
      181 taccgagaac ctgtacttcc aatccatgga aaactttaat aatttctata tacttacatc
      241 taaagagcta gggagaggta aatttgctgt ggttagacaa tgtatatcaa aatctactgg
      301 ccaagaatat gctgcaaaat ttctaaaaaa gagaagaaga ggacaggatt gtcgggcaga
      361 aattttacac gagattgctg tgcttgaatt ggcaaagtct tgtccccgtg ttattaatct
      421 tcatgaggtc tatgaaaata caagtgaaat cattttgata ttggaatatg ctgcaggtgg
      481 agaaattttc agcctgtgtt tacctgagtt ggctgaaatg gtttctgaaa atgatgttat
      541 cagactcatt aaacaaatac ttgaaggagt ttattatcta catcagaata acattgtaca
      601 ccttgattta aagccacaga atatattact gagcagcata taccctctcg gggacattaa
      661 aatagtagat tttggaatgt ctcgaaaaat agggcatgcg tgtgaacttc gggaaatcat
      721 gggaacacca gaatatttag ctccagaaat cctgaactat gatcccatta ccacagcaac
      781 agatatgtgg aatattggta taatagcata tatgttgtta actcacacat caccatttgt
      841 gggagaagat aatcaagaaa catacctcaa tatctctcaa gttaatgtag attatt
//
