LOCUS       sequence-53373-e         853 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GLRX3
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      394..403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(402..422)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(447..464)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             475..497
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    512..536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(536..555)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(537..555)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(639..658)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttcggatccg tatccacctt tactttagcg ctgggtagga aggagccact agatgcatgt
       61 cgctgaactt ttttggtcaa ctctggggca tgtgcaccat ctaatcggtc gattttctga
      121 gaattcttga aaaacagaaa agtgggaaca gagctaattt catatttttc agatacttca
      181 ggaacacctt cagcttccaa cttcacaaat gaaacttgag ggagttcttt agctaactct
      241 gccataactt cgttcatctg tgcacactgt ggagcccatg gtgcccagaa atggaccaca
      301 aggagggact tggctttgag gcgcagcagc tcctcaaact gcccggctga gccgacctcc
      361 tccacggccg ctacagctgc ctcagccgcc cccgccgcca tggattggaa gtacaggttc
      421 tcggtaccca gatctacacc agaagaatga tgatgatgat ggtgcatatg tatatctcct
      481 tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta
      541 tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt
      601 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
      661 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgcgtatggt
      721 ggcaggcccc cgtggccggg gggactgttg gggcgccatc tccttgcatg cacccattcc
      781 tttgcggcgg cgggtgctca acgggcctca aacctactac tggggctgct tcctaatgca
      841 gggaatcgca taa
//
