LOCUS       sequence-53445-e         951 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, 
            Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, 
            Bonneau R, Selbach M, Dieterich C, Landthaler M
  TITLE     The mRNA-Bound Proteome and Its Global Occupancy Profile on 
            Protein-Coding Transcripts.
  JOURNAL   Mol Cell. 2012 Jun 8;46(5):674-90.
  PUBMED    22681889
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2012-06-8"; volume: "46(5)"; pages: "674-90"
COMMENT     Sequence Label: pFRT/TO/HIS/FLAG/HA-CSNK1E
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     48..72
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             172..195
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             199..222
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             223..249
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    265..289
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
       61 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      121 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccat gcaccaccac
      181 caccaccacc accacatgga ctacaaggac gacgatgaca agtaccctta tgacgtgccc
      241 gattacgctt tgaaccagat atcaacaagt ttgtacaaaa aagcaggctc caccatggag
      301 ctacgtgtgg ggaacaagta ccgcctggga cggaagatcg ggagcgggtc cttcggagat
      361 atctacctgg gtgccaacat cgcctctggt gaggaagtcg ccatcaagct ggagtgtgtg
      421 aagacaaagc acccccagct gcacatcgag agcaagttct acaagatgat gcagggtggc
      481 gtggggatcc cgtccatcaa gtggtgcgga gctgagggcg actacaacgt gatggtcatg
      541 gagctgctgg ggcctagcct cgaggacctg ttcaacttct gttcccgcaa attcagcctc
      601 aagacggtgc tgctcttggc cgaccagatg atcagccgca tcgagtatat ccactccaag
      661 aacttcatcc accgggacgt caagcccgac aacttcctca tggggctggg gaagaagggc
      721 aacctggtct acatcatcga cttcggcctg gccaagaagt accgggacgc ccgcacccac
      781 cagcacattc cctaccggga aaacaagaac ctgaccggca cggcccgcta cgcttccatc
      841 aacacgcacc tgggcattga gcaaagccgt cgagatgacc tggagagcct gggctacgtg
      901 ctcatgtact tcaacctggg ctccctgccc tggcaggggc tcaaagcagc c
//
