LOCUS       sequence-53782-e        1006 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Papapetrou EP, Tomishima MJ, Chambers SM, Mica Y, Reed E, Menon J, 
            Tabar V, Mo Q, Studer L, Sadelain M
  TITLE     Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and 
            c-Myc expression for efficient human iPSC induction and 
            differentiation.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Aug 4. 106(31):12759-64.
  PUBMED    19549847
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Aug 4. 106(31):12759-64."
COMMENT     Sequence Label: pLM-mCerulean-cMyc
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(35..545)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(98..116)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    591..708
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tcctcgccct tgctcaccat accggtggat ccccctgggg agagaggtcg gtgattcggt
       61 caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg
      121 cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc
      181 ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc
      241 ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg
      301 cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg
      361 gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc
      421 gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac
      481 gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaacccca
      541 accccgtgga attcgatatc aagcttctcg aggctagtct cgtgatcgat aaaattttga
      601 atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact
      661 attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaagt taaccgatac
      721 cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc accttcttct
      781 tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg aaacgataat
      841 ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa
      901 cctaccaagc ctcctactat cattatgaat aattttatat accacagcca atttgttatg
      961 ttaaaccaat tccacaaact tgcccattta tctaattcca ataatt
//
