LOCUS       sequence-54415-e         784 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Narain Y, Wyttenbach A, Rankin J, Furlong RA, Rubinsztein DC
  TITLE     A molecular investigation of true dominance in Huntington's disease.
  JOURNAL   J Med Genet. 1999 Oct;36(10):739-46.
  PUBMED    10528852
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Med 
            Genet."; date: "1999-10"; volume: "36(10)"; pages: "739-46"
COMMENT     Sequence Label: pHM6-Q23
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             284..301
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(314..332)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(315..332)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(352..369)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    358..582
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(715..734)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggag accatgtacc catacgacgt cccagactac gctggaagct ttatgaaggc
      121 cttcgagtcc ctcaagtcct tccagcagca gcagcagcag cagcagcagc agcagcagca
      181 gcagcagcag cagcagcagc agcagcaaca gccgccaccg ccgccgccgc cgccgccgcc
      241 tcctcagctt cctcagccgc cgaattcgcg gccgctacac gtgcatcatc atcatcatca
      301 ttaagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
      361 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
      421 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
      481 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
      541 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
      601 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
      661 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      721 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      781 gggg
//
