LOCUS       sequence-55533-e         813 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Smits VA, Klompmaker R, Arnaud L, Rijksen G, Nigg EA, Medema RH
  TITLE     Polo-like kinase-1 is a target of the DNA damage checkpoint.
  JOURNAL   Nat Cell Biol. 2000 Sep;2(9):672-6.
  PUBMED    10980711
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2000-09"; volume: "2(9)"; pages: "672-6"
COMMENT     Sequence Label: pRcCMV myc-Plk1 K82R (Nigg LA184)
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             143..172
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttgctta catttgcttc tgacacaact gtgttcacta
      121 gcaacctcaa acagacacca tggagcagaa gctgatctcc gaggaggacc tgaacatgaa
      181 ttcctgcagc ccgggatcca tgagtgctgc agtgactgca gggaagctgg cacgggcacc
      241 ggccgaccct gggaaagccg gggtccccgg agttgcagct cccggagctc cggcggcggc
      301 tccaccggcg aaagagatcc cggaggtcct agtggaccca cgcagccggc ggcgctatgt
      361 gcggggccgc tttttgggca agggcggctt tgccaagtgc ttcgagatct cggacgcgga
      421 caccaaggag gtgttcgcgg gcaggattgt gcctaagtct ctgctgctca agccgcacca
      481 gagggagaag atgtccatgg aaatatccat tcaccgcagc ctcgcccacc agcacgtcgt
      541 aggattccac ggctttttcg aggacaacga cttcgtgttc gtggtgttgg agctctgccg
      601 ccggaggtct ctcctggagc tgcacaagag gaggaaagcc ctgactgagc ctgaggcccg
      661 atactaccta cggcaaattg tgcttggctg ccagtacctg caccgaaacc gagttattca
      721 tcgagacctc aagctgggca accttttcct gaatgaagat ctggaggtga aaatagggga
      781 ttttggactg gcaaccaaag tcgaatatga cgg
//
