LOCUS       sequence-55690-e         815 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Dorn KV, Hughes CE, Rohatgi R
  TITLE     A Smoothened-Evc2 Complex Transduces the Hedgehog Signal at Primary 
            Cilia.
  JOURNAL   Dev Cell. 2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004.
  PUBMED    22981989
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004."
COMMENT     Sequence Label: pCS2 YFP-Smo-Weyer'sAla5
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     36..53
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(260..262,263..265,266..778)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(305..326)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(566..585)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccgccattct gcctggggac gtcggagcaa gcttgattta ggtgacacta tagaatacaa
       61 gctacttgtt ctttttgcag gatcccatcg attcgaattc accatggccg ctggccgccc
      121 cgtgcgtggg cccgagctgg cgccccggag gctgctgcag ttgctgctgc tggtactgct
      181 ggggggcccg ggccgggggg cggccttgag cgggaacgtg accgggcctg ggcctcacag
      241 cgccagcggg agctcgagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      301 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      361 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      421 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg
      481 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      541 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      601 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      661 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
      721 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
      781 cggcagcgtg cagctcgccg accactacca gcaga
//
