LOCUS       sequence-55790-e         954 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Haurwitz RE, Jinek M, Wiedenheft B, Zhou K, Doudna JA
  TITLE     Sequence- and structure-specific RNA processing by a CRISPR 
            endonuclease.
  JOURNAL   Science. 2010 Sep 10;329(5997):1355-8.
  PUBMED    20829488
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2010-09-10"; volume: "329(5997)"; pages: "1355-8"
COMMENT     Sequence Label: pHMGWA-Pa14Csy4
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    60..84
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(104..667)
                     /codon_start=1
                     /product="CRISPR-associated endoribonuclease responsible 
                     for pre-crRNA processing in Pseudomonas aeruginosa 
                     (Haurwitz et al., 2010)"
                     /label=Csy4
                     /translation="MDHYLDIRLRPDPEFPPAQLMSVLFGKLHQALVAQGGDRIGVSFP
                     DLDESRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQ
                     AKSNPERLRRRLMRRHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGP
                     LQVTAEEGGFTCYGLSKGGFVPWF"
     CDS             complement(677..697)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(718..742)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(764..787)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      895..904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg aggtacatca
       61 ccactttgta caagaaagct gggtcggcgc gcccaccctt ttatcagaac cagggaacga
      121 aacctccttt gctcaacccg taacaggtga atcctccttc ctctgccgtc acctgcaacg
      181 gcccgtggcg gatgaagaga cggaagtgct gtccggtgct ctggctgcgt agcgtgacga
      241 agggcaggtc caaggctctc gcgaccgtat cgggaatgcg tttccgagcc tcctcctcac
      301 tcagatcgtg ccggcgcatg agccgccgcc gcaggcgttc cggattgctt ttcgcctgaa
      361 cccgactgac ctgacggtac ggtgtggggt gaggcacgac tgccggttct ccgaattgca
      421 gatggtcccg caacccttcc agccagggcc gggcgagcag ggcacgaagg tcgtccgccg
      481 aggcatgaat gcgcaggcgc tcgcccagcc gggagcggct ttcgtcgagg tcggggaagc
      541 tcacgccgat cctgtccccg ccctgtgcca ccagggcctg gtggagcttg ccgaagagca
      601 cgctcatgag ttgcgccggg ggaaattccg ggtccggtcg caagcgaatg tcgaggtagt
      661 ggtccatggt gaaggagccc tgaaaataca ggttttcctc gagtacggcg gccgcggagc
      721 ctgctttttt gtacaaactt gtgatgtaag atccggtacc agtctgcgcg tctttcaggg
      781 cttcatcgac agtctgacga ccgctggcgg cgtggatcac cgcagtacgc acggcatacc
      841 agaaagcgga catctgcggg atgttcggca tgatttcacc tttctgggcg ttttccatgg
      901 tggcggcata cgtggatctt tcgccaactc ttcctcgtaa gacttcagcg ctac
//
