LOCUS       sequence-56138-e         636 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  TITLE     Stowers Lab Gateway MultiSite plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: L1-10XQUAS-L4
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     148..164
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     148..164
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     169..188
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        169..187
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    219..314
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(354..592)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(386..407)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 cgcggcctcg acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
       61 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
      121 agattttgag acacgggcca gagctgccag gaaacagcta tgaccatgta atacgactca
      181 ctatagggga tatcgcggcc gccctgcagc tggatggcaa ataatgattt tattttgact
      241 gatagtgacc tgttcgttgc aacaaattga taagcaatgc tttcttataa tgccaacttt
      301 gtatagaaaa gttgggtgct gagtccaacc cagtgagacc ccaattccct attcagagtt
      361 ctcttcttgt attcaataat tacttcttgg cagatttcag tagttgcagt tgatttactt
      421 ggttgctggt tacttttaat tgattcactt taacttgcac tttactgcag attgtttagc
      481 ttgttcagct gcgcttgttt atttgcttag ctttcgctta gcgacgtgtt cactttgctt
      541 gtttgaattg aattgtcgct ccgtagacga agcgcctcta tttatactcc ggcgctcgct
      601 agagtctccg ctcgggctgc agcaattcaa ggtcac
//
