LOCUS       sequence-56335-e        1671 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber
            ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC
  TITLE     Exploiting a natural conformational switch to engineer an 
            interleukin-2 'superkine'.
  JOURNAL   Nature. 2012 Mar 25;484(7395):529-33. doi: 10.1038/nature10975.
  PUBMED    22446627
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-25- 25"; volume: "484"; issue: "7395"; 
            pages: "529-33"
COMMENT     Sequence Label: IL2 pMalLP
FEATURES             Location/Qualifiers
     source          1..1671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..78,79..1176)
                     /codon_start=1
                     /gene="malE"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This gene encodes a signal sequence that can direct 
                     MBP to the bacterial periplasm."
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNG
                     LAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAE
                     ITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDK
                     ELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLV
                     DLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPS
                     KPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKD
                     PRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1150..1173
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1225..1248
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt
       61 tccgcctcgg ctctcgccaa aatcgaagaa ggtaaactgg taatctggat taacggcgat
      121 aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa
      181 gtcaccgttg agcatccgga taaactggaa gagaaattcc cacaggttgc ggcaactggc
      241 gatggccctg acattatctt ctgggcacac gaccgctttg gtggctacgc tcaatctggc
      301 ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacctgg
      361 gatgccgtac gttacaacgg caagctgatt gcttacccga tcgctgttga agcgttatcg
      421 ctgatttata acaaagatct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg
      481 ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg
      541 tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc
      601 aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg tctgaccttc
      661 ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc catcgcagaa
      721 gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc atggtccaac
      781 atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa gggtcaacca
      841 tccaaaccgt tcgttggcgt gctgagcgca ggtattaacg ccgccagtcc gaacaaagag
      901 ctggcaaaag agttcctcga aaactatctg ctgactgatg aaggtctgga agcggttaat
      961 aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat
     1021 ccacgtattg ccgccactat ggaaaacgcc cagaaaggtg aaatcatgcc gaacatcccg
     1081 cagatgtccg ctttctggta tgccgtgcgt actgcggtga tcaacgccgc cagcggtcgt
     1141 cagactgtcg atgaagccct gaaagacgcg cagactaatt cgagctcgaa caacaacaac
     1201 aataacaata acaacaacct cgggctggaa gttctgttcc aggggcccgg agccatggga
     1261 gaattcgcac ctacttcaag ttctacaaag aaaacacagc tacaactgga gcatttactt
     1321 ctggatttac agatgatttt gaatggaatt aataattaca agaatcccaa actcaccagg
     1381 atgctcacat ttaagtttta catgcccaag aaggccacag aactgaaaca tcttcagtgt
     1441 ctagaagaag aactcaaacc tctggaggaa gtgctaaatt tagctcaaag caaaaacttt
     1501 cacttaagac ccagggactt aatcagcaat atcaacgtaa tagttctgga actaaaggga
     1561 tctgaaacaa cattcatgtg tgaatatgct gatgagacag caaccattgt agaatttctg
     1621 aacagatgga ttaccttttg tcaaagcatc atctcaacac taactcatca a
//
