LOCUS       sequence-56951-e       10785 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 10785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH054
FEATURES             Location/Qualifiers
     source          1..10785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(240..1031,1032..1100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     863..882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1101..1205)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2245..3435
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(2597..2616)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(3776..3782,3783..3800,3801..3806))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3821..3842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(4062..4721)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4636..4655
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4722..4824)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      5682..6296
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      5773..6304
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      6372..6591
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             6682..7437
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    7440..7690
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             8016..9191
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             9191..10162
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    10235..10708
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 agaacggagt aacctcggtg tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc
       61 ttatccacaa cattttgcgc acggttatgt ggacaaaata cctggttacc caggccgtgc
      121 cggcacgtta accgggctgc atccgatgca agtgtgtcgc tgtcgacgag ctcgcgagct
      181 cgtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aattaattct
      241 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      301 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
      361 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
      421 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
      481 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
      541 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
      601 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
      661 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
      721 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
      781 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
      841 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
      901 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
      961 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1021 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1081 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1141 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1201 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     1261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcccctc agcgcggccg
     1321 ctagcggatc cttaaaaaac catggccgcc tcaaaaggag aagaaacgac aatgggtgtt
     1381 atcaagccag acatgaagat caagcttaaa atggagggta atgttaatgg tcacgccttc
     1441 gtcattgagg gagagggtga aggaaagcca tacgatggaa ctaatactat taacttggaa
     1501 gtgaaggagg gtgccccact tccattctct tatgatatcc ttactacagc cttcgcatat
     1561 ggaaatcgag cattcacaaa atacccagac ggctgtcgag atatgacggt gttcacaaag
     1621 ttccttaaat tttacttttg gttacatatt ttttcttttt gaaaccaaat ctttatcttt
     1681 gtagcacttt cacggtagcg aaacgttagt ttgaatggaa agatgcctgc agacacataa
     1741 agacaccaaa ctctcatcaa tagttccgta aatttttatt gacagaactt attgacggca
     1801 gtggcaggtg tcataaaaaa aaccatgagg gtaataaata atggcaacag ttaaccagct
     1861 ggtacgcaaa ccacgtgctc gcaaagttgc gaaaagcaac gtgcctgcgc tggaagcatg
     1921 cccgcaaaaa cgtggcgtat gtactcgtgt atatactacc actcctaaaa aaccgaactc
     1981 cgcgctgcgt aaagtatgcc gtgttcgtct gactaacggt ttcgaagtga cttcctacat
     2041 cggtggtgaa ggtcacaacc tgcaggagca ctccgtgatc ctgatccgtg gcggtcgtgt
     2101 taaagacctc ccgggtgttc gttaccacac cgtacgtggt gcgcttgact gctccggcgt
     2161 taaagaccgt aagcaggctc gttccaagta tggcgtgaag cgtcctaagg cttaatggtt
     2221 ctccaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc
     2281 accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg
     2341 cgggatatcg tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat
     2401 gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc
     2461 cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc
     2521 acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     2581 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     2641 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     2701 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     2761 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg
     2821 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     2881 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     2941 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
     3001 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
     3061 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc
     3121 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     3181 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     3241 cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg
     3301 ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt
     3361 gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg
     3421 gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagat
     3481 gtcagagatg gagttttgaa aggtgacgtg aagcataaac tgttgttgga aggaggtgga
     3541 catcatcgag tggactttaa gacaatctac agagctaaga aagctgtgaa attgccagac
     3601 tatcactttg tggatcaccg aattgaaatt ttgaaccatg ataaagatta caataaagtg
     3661 actgtgtatg agtcagcagt agctagaaac tcaactgatg gtatggacga gctctacgta
     3721 agcgcttaat aggtaccgcg gccgcgctga ggggctggcc gctcacaatt ccacacaaca
     3781 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3841 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     3901 aatgaatcgg ccaacgcgaa ccccttgcgg ccgcccgggc cgtcgaccaa ttctcatgtt
     3961 tgacagctta tcatcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag
     4021 caaccaggcg tttaagggca ccaataactg ccttaaaaaa attacgcccc gccctgccac
     4081 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     4141 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     4201 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     4261 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     4321 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     4381 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     4441 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     4501 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     4561 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     4621 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     4681 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     4741 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     4801 ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc ccagggcttc
     4861 ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc cgtcacaggt
     4921 atttattcgc gataagctca tggagcggcg taaccgtcgc acaggaagga cagagaaagc
     4981 gcggatctgg gaagtgacgg acagaacggt caggacctgg attggggagg cggttgccgc
     5041 cgctgctgct gacggtgtga cgttctctgt tccggtcaca ccacatacgt tccgccattc
     5101 ctatgcgatg cacatgctgt atgccggtat accgctgaaa gttctgcaaa gcctgatggg
     5161 acataagtcc atcagttcaa cggaagtcta cacgaaggtt tttgcgctgg atgtggctgc
     5221 ccggcaccgg gtgcagtttg cgatgccgga gtctgatgcg gttgcgatgc tgaaacaatt
     5281 atcctgagaa taaatgcctt ggcctttata tggaaatgtg gaactgagtg gatatgctgt
     5341 ttttgtctgt taaacagaga agctggctgt tatccactga gaagcgaacg aaacagtcgg
     5401 gaaaatctcc cattatcgta gagatccgca ttattaatct caggagcctg tgtagcgttt
     5461 ataggaagta gtgttctgtc atgatgcctg caagcggtaa cgaaaacgat ttgaatatgc
     5521 cttcaggaac aatagaaatc ttcgtgcggt gttacgttga agtggagcgg attatgtcag
     5581 caatggacag aacaacctaa tgaacacaga accatgatgt ggtctgtcct tttacagcca
     5641 gtagtgctcg ccgcagtcga gcgacagggc gaagccctcg gctggttgcc ctcgccgctg
     5701 ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga
     5761 gacaccgcgg ccggccgccg gcgttgtgga tacctcgcgg aaaacttggc cctcactgac
     5821 agatgagggg cggacgttga cacttgaggg gccgactcac ccggcgcggc gttgacagat
     5881 gaggggcagg ctcgatttcg gccggcgacg tggagctggc cagcctcgca aatcggcgaa
     5941 aacgcctgat tttacgcgag tttcccacag atgatgtgga caagcctggg gataagtgcc
     6001 ctgcggtatt gacacttgag gggcgcgact actgacagat gaggggcgcg atccttgaca
     6061 cttgaggggc agagtgctga cagatgaggg gcgcacctat tgacatttga ggggctgtcc
     6121 acaggcagaa aatccagcat ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct
     6181 gtcttttaac ctgcttttaa accaatattt ataaaccttg tttttaacca gggctgcgcc
     6241 ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggtc
     6301 gagtgagcga ggaagcacca gggaacagca cttatatatt ctgcttacac acgatgcctg
     6361 aaaaaacttc ccttggggtt atccacttat ccacggggat atttttataa ttattttttt
     6421 tatagttttt agatcttctt ttttagagcg ccttgtaggc ctttatccat gctggttcta
     6481 gagaaggtgt tgtgacaaat tgccctttca gtgtgacaaa tcaccctcaa atgacagtcc
     6541 tgtctgtgac aaattgccct taaccctgtg acaaattgcc ctcagaagaa gctgtttttt
     6601 cacaaagtta tccctgctta ttgactcttt tttatttagt gtgacaatct aaaaacttgt
     6661 cacacttcac atggatctgt catggcggaa acagcggtta tcaatcacaa gaaacgtaaa
     6721 aatagcccgc gaatcgtcca gtcaaacgac ctcactgagg cggcatatag tctctcccgg
     6781 gatcaaaaac gtatgctgta tctgttcgtt gaccagatca gaaaatctga tggcacccta
     6841 caggaacatg acggtatctg cgagatccat gttgctaaat atgctgaaat attcggattg
     6901 acctctgcgg aagccagtaa ggatatacgg caggcattga agagtttcgc ggggaaggaa
     6961 gtggtttttt atcgccctga agaggatgcc ggcgatgaaa aaggctatga atcttttcct
     7021 tggtttatca aacgtgcgca cagtccatcc agagggcttt acagtgtaca tatcaaccca
     7081 tatctcattc ccttctttat cgggttacag aaccggttta cgcagtttcg gcttagtgaa
     7141 acaaaagaaa tcaccaatcc gtatgccatg cgtttatacg aatccctgtg tcagtatcgt
     7201 aagccggatg gctcaggcat cgtctctctg aaaatcgact ggatcataga gcgttaccag
     7261 ctgcctcaaa gttaccagcg tatgcctgac ttccgccgcc gcttcctgca ggtctgtgtt
     7321 aatgagatca acagcagaac tccaatgcgc ctctcataca ttgagaaaaa gaaaggccgc
     7381 cagacgactc atatcgtatt ttccttccgc gatatcactt ccatgacgac aggatagtct
     7441 gagggttatc tgtcacagat ttgagggtgg ttcgtcacat ttgttctgac ctactgaggg
     7501 taatttgtca cagttttgct gtttccttca gcctgcatgg attttctcat actttttgaa
     7561 ctgtaatttt taaggaagcc aaatttgagg gcagtttgtc acagttgatt tccttctctt
     7621 tcccttcgtc atgtgacctg atatcggggg ttagttcgtc atcattgatg agggttgatt
     7681 atcacagttt attactctga attggctatc cgcgtgtgta cctctacctg gagtttttcc
     7741 cacggtggat atttcttctt gcgctgagcg taagagctat ctgacagaac agttcttctt
     7801 tgcttcctcg ccagttcgct cgctatgctc ggttacacgg ctgcggcgag cgctagtgat
     7861 aataagtgac tgaggtatgt gctcttctta tctccttttg tagtgttgct cttattttaa
     7921 acaactttgc ggttttttga tgactttgcg attttgttgt tgctttgcag taaattgcaa
     7981 gatttaataa aaaaacgcaa agcaatgatt aaaggatgtt cagaatgaaa ctcatggaaa
     8041 cacttaacca gtgcataaac gctggtcatg aaatgacgaa ggctatcgcc attgcacagt
     8101 ttaatgatga cagcccggaa gcgaggaaaa taacccggcg ctggagaata ggtgaagcag
     8161 cggatttagt tggggtttct tctcaggcta tcagagatgc cgagaaagca gggcgactac
     8221 cgcacccgga tatggaaatt cgaggacggg ttgagcaacg tgttggttat acaattgaac
     8281 aaattaatca tatgcgtgat gtgtttggta cgcgattgcg acgtgctgaa gacgtatttc
     8341 caccggtgat cggggttgct gcccataaag gtggcgttta caaaacctca gtttctgttc
     8401 atcttgctca ggatctggct ctgaaggggc tacgtgtttt gctcgtggaa ggtaacgacc
     8461 cccagggaac agcctcaatg tatcacggat gggtaccaga tcttcatatt catgcagaag
     8521 acactctcct gcctttctat cttggggaaa aggacgatgt cacttatgca ataaagccca
     8581 cttgctggcc ggggcttgac attattcctt cctgtctggc tctgcaccgt attgaaactg
     8641 agttaatggg caaatttgat gaaggtaaac tgcccaccga tccacacctg atgctccgac
     8701 tggccattga aactgttgct catgactatg atgtcatagt tattgacagc gcgcctaacc
     8761 tgggtatcgg cacgattaat gtcgtatgtg ctgctgatgt gctgattgtt cccacgcctg
     8821 ctgagttgtt tgactacacc tccgcactgc agtttttcga tatgcttcgt gatctgctca
     8881 agaacgttga tcttaaaggg ttcgagcctg atgtacgtat tttgcttacc aaatacagca
     8941 atagtaatgg ctctcagtcc ccgtggatgg aggagcaaat tcgggatgcc tggggaagca
     9001 tggttctaaa aaatgttgta cgtgaaacgg atgaagttgg taaaggtcag atccggatga
     9061 gaactgtttt tgaacaggcc attgatcaac gctcttcaac tggtgcctgg agaaatgctc
     9121 tttctatttg ggaacctgtc tgcaatgaaa ttttcgatcg tctgattaaa ccacgctggg
     9181 agattagata atgaagcgtg cgcctgttat tccaaaacat acgctcaata ctcaaccggt
     9241 tgaagatact tcgttatcga caccagctgc cccgatggtg gattcgttaa ttgcgcgcgt
     9301 aggagtaatg gctcgcggta atgccattac tttgcctgta tgtggtcggg atgtgaagtt
     9361 tactcttgaa gtgctccggg gtgatagtgt tgagaagacc tctcgggtat ggtcaggtaa
     9421 tgaacgtgac caggagctgc ttactgagga cgcactggat gatctcatcc cttcttttct
     9481 actgactggt caacagacac cggcgttcgg tcgaagagta tctggtgtca tagaaattgc
     9541 cgatgggagt cgccgtcgta aagctgctgc acttaccgaa agtgattatc gtgttctggt
     9601 tggcgagctg gatgatgagc agatggctgc attatccaga ttgggtaacg attatcgccc
     9661 aacaagtgct tatgaacgtg gtcagcgtta tgcaagccga ttgcagaatg aatttgctgg
     9721 aaatatttct gcgctggctg atgcggaaaa tatttcacgt aagattatta cccgctgtat
     9781 caacaccgcc aaattgccta aatcagttgt tgctcttttt tctcaccccg gtgaactatc
     9841 tgcccggtca ggtgatgcac ttcaaaaagc ctttacagat aaagaggaat tacttaagca
     9901 gcaggcatct aaccttcatg agcagaaaaa agctggggtg atatttgaag ctgaagaagt
     9961 tatcactctt ttaacttctg tgcttaaaac gtcatctgca tcaagaacta gtttaagctc
    10021 acgacatcag tttgctcctg gagcgacagt attgtataag ggcgataaaa tggtgcttaa
    10081 cctggacagg tctcgtgttc caactgagtg tatagagaaa attgaggcca ttcttaagga
    10141 acttgaaaag ccagcaccct gatgcgacca cgttttagtc tacgtttatc tgtctttact
    10201 taatgtcctt tgttacaggc cagaaagcat aactggcctg aatattctct ctgggcccac
    10261 tgttccactt gtatcgtcgg tctgataatc agactgggac cacggtccca ctcgtatcgt
    10321 cggtctgatt attagtctgg gaccacggtc ccactcgtat cgtcggtctg attattagtc
    10381 tgggaccacg gtcccactcg tatcgtcggt ctgataatca gactgggacc acggtcccac
    10441 tcgtatcgtc ggtctgatta ttagtctggg accatggtcc cactcgtatc gtcggtctga
    10501 ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgattattag tctggaacca
    10561 cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacggtccc actcgtatcg
    10621 tcggtctgat tattagtctg ggaccacgat cccactcgtg ttgtcggtct gattatcggt
    10681 ctgggaccac ggtcccactt gtattgtcga tcagactatc agcgtgagac tacgattcca
    10741 tcaatgcctg tcaagggcaa gtattgacat gtcgtcgtaa cctgt
//
