LOCUS       sequence-56959-e        9297 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT
  TITLE     A simplified counter-selection recombineering protocol for creating 
            fluorescent protein reporter constructs directly from C. elegans 
            fosmid genomic clones.
  JOURNAL   BMC Biotechnol. 2013 Jan 3;13(1):1.
  PUBMED    23281894
REFERENCE   2  (bases 1 to 9297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-01-3"; volume: "13(1)"; pages: "1"
COMMENT     Sequence Label: pNH058
FEATURES             Location/Qualifiers
     source          1..9297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(240..1031,1032..1100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     863..882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1101..1205)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1348..1392
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             1408..1431
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1447..1470
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     promoter        complement(join(2288..2294,2295..2312,2313..2318))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2333..2354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(2574..3233)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3148..3167
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3234..3336)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      4194..4808
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4285..4816
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4884..5103
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             5194..5949
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    5952..6202
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             6528..7703
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             7703..8674
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    8747..9220
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 agaacggagt aacctcggtg tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc
       61 ttatccacaa cattttgcgc acggttatgt ggacaaaata cctggttacc caggccgtgc
      121 cggcacgtta accgggctgc atccgatgca agtgtgtcgc tgtcgacgag ctcgcgagct
      181 cgtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aattaattct
      241 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
      301 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
      361 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
      421 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
      481 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
      541 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
      601 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
      661 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
      721 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
      781 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
      841 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
      901 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
      961 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     1021 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     1081 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1141 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     1201 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     1261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcccctc agcgcggccg
     1321 ctagcggatc cttaaaaaac catggttaaa gaaacagcag cagcgaaatt tgaaagacaa
     1381 catatggata gtggaggcgg tggaagtttg gaagttcttt tccaaggacc gggcggagga
     1441 ggctcttggt cgcatccaca gttcgaaaaa gccatggccg gcggtggtgg atcgggagga
     1501 ggaggttcgg gtggcggagg atcgggcgcc tcaaaaggag aagaaacgac aatgggtgtt
     1561 atcaagccag acatgaagat caagcttaaa atggagggta atgttaatgg tcacgccttc
     1621 gtcattgagg gagagggtga aggaaagcca tacgatggaa ctaatactat taacttggaa
     1681 gtgaaggagg gtgccccact tccattctct tatgatatcc ttactacagc cttcgcatat
     1741 ggaaatcgag cattcacaaa atacccagac gacattccaa attacttcaa gcagtcattt
     1801 ccagagggat attcttggga gagaacaatg acattcgagg acaagggtat agttaaagtg
     1861 aaatctgata ttagtatgga agaagattca ttcatctatg agatacacct gaagggcgag
     1921 aactttcctc cgaatggacc agtgatgcag aaaaagacaa caggatggga cgcaagtact
     1981 gaacgtatgt atgtcagaga tggagttttg aaaggtgacg tgaagcataa actgttgttg
     2041 gaaggaggtg gacatcatcg agtggacttt aagacaatct acagagctaa gaaagctgtg
     2101 aaattgccag actatcactt tgtggatcac cgaattgaaa ttttgaacca tgataaagat
     2161 tacaataaag tgactgtgta tgagtcagca gtagctagaa actcaactga tggtatggac
     2221 gagctctacg taagcgctta ataggtaccg cggccgcgct gaggggctgg ccgctcacaa
     2281 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2341 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     2401 gccagctgca ttaatgaatc ggccaacgcg aaccccttgc ggccgcccgg gccgtcgacc
     2461 aattctcatg tttgacagct tatcatcgaa tttctgccat tcatccgctt attatcactt
     2521 attcaggcgt agcaaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc
     2581 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     2641 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     2701 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     2761 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     2821 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     2881 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     2941 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     3001 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     3061 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     3121 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     3181 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     3241 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     3301 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg
     3361 gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct
     3421 tccgtcacag gtatttattc gcgataagct catggagcgg cgtaaccgtc gcacaggaag
     3481 gacagagaaa gcgcggatct gggaagtgac ggacagaacg gtcaggacct ggattgggga
     3541 ggcggttgcc gccgctgctg ctgacggtgt gacgttctct gttccggtca caccacatac
     3601 gttccgccat tcctatgcga tgcacatgct gtatgccggt ataccgctga aagttctgca
     3661 aagcctgatg ggacataagt ccatcagttc aacggaagtc tacacgaagg tttttgcgct
     3721 ggatgtggct gcccggcacc gggtgcagtt tgcgatgccg gagtctgatg cggttgcgat
     3781 gctgaaacaa ttatcctgag aataaatgcc ttggccttta tatggaaatg tggaactgag
     3841 tggatatgct gtttttgtct gttaaacaga gaagctggct gttatccact gagaagcgaa
     3901 cgaaacagtc gggaaaatct cccattatcg tagagatccg cattattaat ctcaggagcc
     3961 tgtgtagcgt ttataggaag tagtgttctg tcatgatgcc tgcaagcggt aacgaaaacg
     4021 atttgaatat gccttcagga acaatagaaa tcttcgtgcg gtgttacgtt gaagtggagc
     4081 ggattatgtc agcaatggac agaacaacct aatgaacaca gaaccatgat gtggtctgtc
     4141 cttttacagc cagtagtgct cgccgcagtc gagcgacagg gcgaagccct cggctggttg
     4201 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga
     4261 agccgtgtgc gagacaccgc ggccggccgc cggcgttgtg gatacctcgc ggaaaacttg
     4321 gccctcactg acagatgagg ggcggacgtt gacacttgag gggccgactc acccggcgcg
     4381 gcgttgacag atgaggggca ggctcgattt cggccggcga cgtggagctg gccagcctcg
     4441 caaatcggcg aaaacgcctg attttacgcg agtttcccac agatgatgtg gacaagcctg
     4501 gggataagtg ccctgcggta ttgacacttg aggggcgcga ctactgacag atgaggggcg
     4561 cgatccttga cacttgaggg gcagagtgct gacagatgag gggcgcacct attgacattt
     4621 gaggggctgt ccacaggcag aaaatccagc atttgcaagg gtttccgccc gtttttcggc
     4681 caccgctaac ctgtctttta acctgctttt aaaccaatat ttataaacct tgtttttaac
     4741 cagggctgcg ccctgtgcgc gtgaccgcgc acgccgaagg ggggtgcccc cccttctcga
     4801 accctcccgg tcgagtgagc gaggaagcac cagggaacag cacttatata ttctgcttac
     4861 acacgatgcc tgaaaaaact tcccttgggg ttatccactt atccacgggg atatttttat
     4921 aattattttt tttatagttt ttagatcttc ttttttagag cgccttgtag gcctttatcc
     4981 atgctggttc tagagaaggt gttgtgacaa attgcccttt cagtgtgaca aatcaccctc
     5041 aaatgacagt cctgtctgtg acaaattgcc cttaaccctg tgacaaattg ccctcagaag
     5101 aagctgtttt ttcacaaagt tatccctgct tattgactct tttttattta gtgtgacaat
     5161 ctaaaaactt gtcacacttc acatggatct gtcatggcgg aaacagcggt tatcaatcac
     5221 aagaaacgta aaaatagccc gcgaatcgtc cagtcaaacg acctcactga ggcggcatat
     5281 agtctctccc gggatcaaaa acgtatgctg tatctgttcg ttgaccagat cagaaaatct
     5341 gatggcaccc tacaggaaca tgacggtatc tgcgagatcc atgttgctaa atatgctgaa
     5401 atattcggat tgacctctgc ggaagccagt aaggatatac ggcaggcatt gaagagtttc
     5461 gcggggaagg aagtggtttt ttatcgccct gaagaggatg ccggcgatga aaaaggctat
     5521 gaatcttttc cttggtttat caaacgtgcg cacagtccat ccagagggct ttacagtgta
     5581 catatcaacc catatctcat tcccttcttt atcgggttac agaaccggtt tacgcagttt
     5641 cggcttagtg aaacaaaaga aatcaccaat ccgtatgcca tgcgtttata cgaatccctg
     5701 tgtcagtatc gtaagccgga tggctcaggc atcgtctctc tgaaaatcga ctggatcata
     5761 gagcgttacc agctgcctca aagttaccag cgtatgcctg acttccgccg ccgcttcctg
     5821 caggtctgtg ttaatgagat caacagcaga actccaatgc gcctctcata cattgagaaa
     5881 aagaaaggcc gccagacgac tcatatcgta ttttccttcc gcgatatcac ttccatgacg
     5941 acaggatagt ctgagggtta tctgtcacag atttgagggt ggttcgtcac atttgttctg
     6001 acctactgag ggtaatttgt cacagttttg ctgtttcctt cagcctgcat ggattttctc
     6061 atactttttg aactgtaatt tttaaggaag ccaaatttga gggcagtttg tcacagttga
     6121 tttccttctc tttcccttcg tcatgtgacc tgatatcggg ggttagttcg tcatcattga
     6181 tgagggttga ttatcacagt ttattactct gaattggcta tccgcgtgtg tacctctacc
     6241 tggagttttt cccacggtgg atatttcttc ttgcgctgag cgtaagagct atctgacaga
     6301 acagttcttc tttgcttcct cgccagttcg ctcgctatgc tcggttacac ggctgcggcg
     6361 agcgctagtg ataataagtg actgaggtat gtgctcttct tatctccttt tgtagtgttg
     6421 ctcttatttt aaacaacttt gcggtttttt gatgactttg cgattttgtt gttgctttgc
     6481 agtaaattgc aagatttaat aaaaaaacgc aaagcaatga ttaaaggatg ttcagaatga
     6541 aactcatgga aacacttaac cagtgcataa acgctggtca tgaaatgacg aaggctatcg
     6601 ccattgcaca gtttaatgat gacagcccgg aagcgaggaa aataacccgg cgctggagaa
     6661 taggtgaagc agcggattta gttggggttt cttctcaggc tatcagagat gccgagaaag
     6721 cagggcgact accgcacccg gatatggaaa ttcgaggacg ggttgagcaa cgtgttggtt
     6781 atacaattga acaaattaat catatgcgtg atgtgtttgg tacgcgattg cgacgtgctg
     6841 aagacgtatt tccaccggtg atcggggttg ctgcccataa aggtggcgtt tacaaaacct
     6901 cagtttctgt tcatcttgct caggatctgg ctctgaaggg gctacgtgtt ttgctcgtgg
     6961 aaggtaacga cccccaggga acagcctcaa tgtatcacgg atgggtacca gatcttcata
     7021 ttcatgcaga agacactctc ctgcctttct atcttgggga aaaggacgat gtcacttatg
     7081 caataaagcc cacttgctgg ccggggcttg acattattcc ttcctgtctg gctctgcacc
     7141 gtattgaaac tgagttaatg ggcaaatttg atgaaggtaa actgcccacc gatccacacc
     7201 tgatgctccg actggccatt gaaactgttg ctcatgacta tgatgtcata gttattgaca
     7261 gcgcgcctaa cctgggtatc ggcacgatta atgtcgtatg tgctgctgat gtgctgattg
     7321 ttcccacgcc tgctgagttg tttgactaca cctccgcact gcagtttttc gatatgcttc
     7381 gtgatctgct caagaacgtt gatcttaaag ggttcgagcc tgatgtacgt attttgctta
     7441 ccaaatacag caatagtaat ggctctcagt ccccgtggat ggaggagcaa attcgggatg
     7501 cctggggaag catggttcta aaaaatgttg tacgtgaaac ggatgaagtt ggtaaaggtc
     7561 agatccggat gagaactgtt tttgaacagg ccattgatca acgctcttca actggtgcct
     7621 ggagaaatgc tctttctatt tgggaacctg tctgcaatga aattttcgat cgtctgatta
     7681 aaccacgctg ggagattaga taatgaagcg tgcgcctgtt attccaaaac atacgctcaa
     7741 tactcaaccg gttgaagata cttcgttatc gacaccagct gccccgatgg tggattcgtt
     7801 aattgcgcgc gtaggagtaa tggctcgcgg taatgccatt actttgcctg tatgtggtcg
     7861 ggatgtgaag tttactcttg aagtgctccg gggtgatagt gttgagaaga cctctcgggt
     7921 atggtcaggt aatgaacgtg accaggagct gcttactgag gacgcactgg atgatctcat
     7981 cccttctttt ctactgactg gtcaacagac accggcgttc ggtcgaagag tatctggtgt
     8041 catagaaatt gccgatggga gtcgccgtcg taaagctgct gcacttaccg aaagtgatta
     8101 tcgtgttctg gttggcgagc tggatgatga gcagatggct gcattatcca gattgggtaa
     8161 cgattatcgc ccaacaagtg cttatgaacg tggtcagcgt tatgcaagcc gattgcagaa
     8221 tgaatttgct ggaaatattt ctgcgctggc tgatgcggaa aatatttcac gtaagattat
     8281 tacccgctgt atcaacaccg ccaaattgcc taaatcagtt gttgctcttt tttctcaccc
     8341 cggtgaacta tctgcccggt caggtgatgc acttcaaaaa gcctttacag ataaagagga
     8401 attacttaag cagcaggcat ctaaccttca tgagcagaaa aaagctgggg tgatatttga
     8461 agctgaagaa gttatcactc ttttaacttc tgtgcttaaa acgtcatctg catcaagaac
     8521 tagtttaagc tcacgacatc agtttgctcc tggagcgaca gtattgtata agggcgataa
     8581 aatggtgctt aacctggaca ggtctcgtgt tccaactgag tgtatagaga aaattgaggc
     8641 cattcttaag gaacttgaaa agccagcacc ctgatgcgac cacgttttag tctacgttta
     8701 tctgtcttta cttaatgtcc tttgttacag gccagaaagc ataactggcc tgaatattct
     8761 ctctgggccc actgttccac ttgtatcgtc ggtctgataa tcagactggg accacggtcc
     8821 cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt atcgtcggtc
     8881 tgattattag tctgggacca cggtcccact cgtatcgtcg gtctgataat cagactggga
     8941 ccacggtccc actcgtatcg tcggtctgat tattagtctg ggaccatggt cccactcgta
     9001 tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgattatt
     9061 agtctggaac cacggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc
     9121 ccactcgtat cgtcggtctg attattagtc tgggaccacg atcccactcg tgttgtcggt
     9181 ctgattatcg gtctgggacc acggtcccac ttgtattgtc gatcagacta tcagcgtgag
     9241 actacgattc catcaatgcc tgtcaagggc aagtattgac atgtcgtcgt aacctgt
//
